Gastric epithelial morphogenesis in normal and transgenic mice

The American Journal of Physiology
S M KaramJ I Gordon

Abstract

The epithelium located in the corpus of the adult mouse stomach forms mucosal invaginations known as gastric units. Gastric units are populated by members of the pit, parietal, and neck-zymogenic cell lineages all of which are derived from multipotent stem cells. Gastric unit morphogenesis was examined in normal embryonic day 18 (E18) to postnatal day 28 (P28) FVB/N mice with electron microscopy and multilabel immunohistochemistry. E18 units appear as short, solid infoldings (primordial buds), 92% of whose cells represent pit, parietal, and neck cell precursors. Although the total number of cells per bud does not change from P1 to P7, immature cells decrease to 22% as differentiated pit, neck, and parietal cells appear. From P7 to P15, lineage precursors and their differentiated progeny increase and buds elongate. Between P15 and P21 the multipotent stem cell and its descendants are assembled into a distinct proliferative zone (isthmus) located in the midportion of each unit, and cellular migration-differentiation programs become compartmentalized. To examine the role of parietal cells in regulating gastric unit morphogenesis, nucleotides -1035 to +24 of the mouse H(+)-K(+)-adenosinetriphosphatase beta-subunit gene were used to...Continue Reading

Citations

Aug 29, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·W KongR H Diamond
Jan 9, 2003·Annual Review of Physiology·Linda C Samuelson, Karen L Hinkle
Mar 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jung D OhJeffrey I Gordon
Sep 4, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Theresa M Keeley, Linda C Samuelson
Oct 12, 2007·Physiological Reviews·Gijs R van den Brink
Oct 22, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Karen L HinkleLinda C Samuelson
Nov 14, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·T V FranicI R Van Driel
Jul 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·A PaglioccaA Varro
Apr 28, 2018·Journal of Cell Science·Meina HuangXinhua Lin

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