Gastric GATA-6 DNA-binding protein: proteolysis induced by cAMP

FEBS Letters
R NakagawaM Maeda

Abstract

The rat gastric GATA DNA-binding protein, GATA-6 (GATA-GT1), was stably expressed in CHO-K1 cells. The GATA-6 protein was localized in the nucleus but not in the cytoplasm. Interestingly, when cells were treated with dibutyryl cAMP, the GATA-6 protein was specifically degraded. Such a phenomenon was not observed in the presence of 5'-AMP or dibutyryl cGMP. The cellular level of the GATA-6 protein was restored upon removal of dibutyryl cAMP. Degradation was also induced by cholera toxin, which increased the cellular cAMP concentration, and was inhibited by a protein kinase A inhibitor. However, activators of protein kinase C did not have any effect. The degradation was inhibited by proteasome inhibitors (PSI (benzyloxycarbonyl-Ile-Glu(O-t-Bu)-Ala-leucinal) and MG115 (benzyloxycarbonyl-Leu-Leu-norvalinal)) but not by those of lysosomes and serine proteases. These results suggest that a kinase-mediated protein phosphorylation is the cellular signal for degradation of the GATA-6 protein. This finding constitutes a novel aspect of regulation by GATA DNA-binding proteins, which are essential for developmental processes and tissue-specific transcription.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1992·The Journal of Cell Biology·N D RidgwayJ L Goldstein
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A CiechanoverG M Brodeur
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L I ZonS H Orkin
Jan 1, 1986·Annual Review of Biochemistry·I MellmanA Helenius
May 16, 1995·Biochemical and Biophysical Research Communications·T NishiM Futai
Feb 1, 1995·Digestive Diseases and Sciences·B I HirschowitzG Sachs
Feb 24, 1995·Cell·D Thanos, T Maniatis
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S TamuraM Futai

❮ Previous
Next ❯

Citations

May 1, 2001·Biochemical and Biophysical Research Communications·R NakagawaM Maeda
May 26, 2005·Biochemical and Biophysical Research Communications·Akiko IshidaMasatomo Maeda
Dec 1, 2005·Development, Growth & Differentiation·Masatomo MaedaAyako Ohashi-Kobayashi
Apr 20, 2005·Regulatory Peptides·Rana Temsah, Mona Nemer
Jun 15, 2012·Biochemical and Biophysical Research Communications·Hironori Ushijima, Masatomo Maeda
Oct 24, 1998·Biochemical and Biophysical Research Communications·Y SakaiM Maeda
Feb 22, 2005·Biochemical and Biophysical Research Communications·Masatomo MaedaAyako Kobayashi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.