Gastric mucosal density of Helicobacter pylori estimated by real-time PCR compared with results of urea breath test and histological grading

Journal of Medical Microbiology
D KobayashiM Koike

Abstract

The accuracy of the urea breath test (UBT) and histological grading for estimation of the density of Helicobacter pylori in gastric mucosa is not known. Real-time (TaqMan) PCR was used to estimate the total number of H. pylori genomes in biopsy samples. These values were compared with those obtained by the UBT and the histological grade obtained by the Sydney system. The UBT and endoscopy with antral and corporal biopsies were performed in 88 consecutive untreated patients with dyspepsia. Bacterial culture and the rapid urease test were done with fresh biopsy materials. TaqMan PCR and histological examination were done on serial paraffin sections of the biopsy samples. Of the five methods tested, TaqMan PCR had the highest sensitivity and specificity (both 100%) in the diagnosis of H. pylori infection. The mean density of H. pylori genomes for pairs of biopsy samples from individual patients was compared with the individual values obtained by the UBT; correlation between the results was significant. The density of H. pylori genomes was higher in histological grades 1, 2 and 3 than in grade 0, without significant differences between adjacent grades from 1 to 3. These results suggest that the severity of H. pylori infection of th...Continue Reading

Citations

Jan 26, 2011·European Journal of Clinical Investigation·Noam ZevitRaanan Shamir
Jul 21, 2010·American Journal of Hematology·Didier DecaudinMarc Lecuit
Dec 22, 2005·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Masumi OkudaIchiro Imoto
Nov 29, 2005·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·J P GisbertJ M Pajares
Nov 11, 2005·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·J P Gisbert, J M Pajares
Jan 1, 2010·Journal of Zhejiang University. Science. B·Leonardo Martín PérezJordi Morató
Dec 18, 2013·Marine Pollution Bulletin·Chelsea B HolmanAdesh Ramsubhag
Apr 23, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Sreenivasulu PuramVenkateswarlu Kudiganti
Nov 5, 2015·United European Gastroenterology Journal·María J Ramírez-LázaroFerran Segura
Mar 16, 2018·The Cochrane Database of Systematic Reviews·Lawrence Mj BestKurinchi Selvan Gurusamy
Jun 28, 2012·World Journal of Gastrointestinal Pathophysiology·Menachem MoshkowitzErwin Santo
Aug 26, 2006·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Eugenia AriasGustavo Sevlever
Apr 22, 2005·Scandinavian Journal of Gastroenterology·Monica FrancoFabio Farinati
Jun 29, 2007·Digestive Diseases and Sciences·Angelo ZulloDino Vaira
Jul 8, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·N SainsusR Hofmann-Lehmann
Aug 29, 2009·Digestive Diseases and Sciences·Ahmet TuzunAhmet Uygun
Jan 26, 2006·The American Journal of Surgical Pathology·Veronika ZsiklaGieri Cathomas
May 25, 2013·Journal of Gastroenterology and Hepatology·Toshihiro NishizawaToshifumi Hibi
Nov 21, 2018·Foodborne Pathogens and Disease·Maria Consuelo Pina-PérezMaria Antonia Ferrús
Oct 24, 2002·Journal of Clinical Gastroenterology·John K Marshall, Deborah L R Yamamura
Jun 1, 2011·PloS One·María José Ramírez-LázaroXavier Calvet
Sep 21, 2004·Journal of Gastroenterology and Hepatology·Angelo ZulloSergio Morini
Dec 20, 2011·Digestive Diseases·M Varbanova, P Malfertheiner
Jun 6, 2003·Alimentary Pharmacology & Therapeutics·C BassetD Vaira
Nov 18, 2003·Helicobacter·Hilpi RautelinFrancis Mégraud
Feb 4, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Joanne L Simala-Grant, Diane E Taylor
Feb 1, 2017·Jundishapur Journal of Microbiology·Mitra Samareh-FekriArshia Jamali Paghalhe
Jan 1, 2014·Qatar Medical Journal·Ebtisam E Al-MoayadNezar N Al-Hebshi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.