Gastric mucosal toxicity of duodenal juice constituents in the rat. Acute studies using ex vivo rat gastric chamber model

Digestive Diseases and Sciences
D ArmstrongR H Dowling

Abstract

To determine the acute gastrotoxicity of refluxed duodenal contents, an ex vivo rat gastric chamber was used to study mucosal damage produced by conjugated and unconjugated human bile acids and lysolecithin at neutral and acidic pH; the effects of trypsin, human duodenal aspirate, and combinations of chenodeoxycholic acid, lecithin, and lysolecithin were also studied. At neutral pH, all bile acids except tauroursodeoxycholic acid, caused dose-dependent falls in mucosal potential difference and losses of mucosal nucleic acid into the chamber fluid, indicating mucosal damage. The di-alpha-hydroxy bile acids, deoxycholic and chenodeoxycholic acids, were more gastrotoxic than cholic and ursodeoxycholic acids, and all unconjugated bile acids were more toxic than their conjugated species, none of which produced damage at concentrations below 2.0 mM. For all but the taurine conjugates, bile acid-induced changes in potential difference were lower at acidic then at neutral pH. Lysolecithin gastrotoxicity, comparable at neutral pH to that of chenodeoxycholic acid, was also reduced at acidic pH. Lecithin decreased the gastrotoxicity of chenodeoxycholic acid and lysolecithin. Trypsin produced no damage, and the gastrotoxicity of human duod...Continue Reading

References

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Citations

Sep 9, 2010·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Joerg ZehetnerTom R DeMeester
Apr 2, 2013·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Herbert L Dupont
Apr 28, 2009·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·Philip Wy ChiuTom R DeMeester
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Mar 31, 2015·Annals of Surgery·Christina L GreeneTom R DeMeester

Related Concepts

Metazoa
Bile Salts
Dose-Response Relationship, Drug
Duodenum
Structure of Pyloric Gland
Hydrogen-Ion Concentration
Intestinal Secretions
Resting Potentials
Rats, Wistar
Rats, Laboratory

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