PMID: 7524350Oct 1, 1994Paper

Gastrin effects on isolated rat enterochromaffin-like cells in primary culture

The American Journal of Physiology
Christian PrinzG Sachs

Abstract

The hormone gastrin stimulates acid secretion by releasing histamine from gastric enterochromaffin-like (ECL) cells and induces ECL cell proliferation in vivo. This study uses a > 90% pure ECL cell preparation in culture to compare gastrin effects on histamine release, histidine decarboxylase (HDC) activity, and DNA synthesis. Gastrin and the cholecystokinin octapeptide (CCK-8, nonsulfated) induced histamine release from ECL cells (24-96 h of primary culture) within 5 min of incubation [concentration eliciting 50% of maximal response (EC50), 4 and 2 x 10(-11) M, respectively]. The CCK-B antagonist L-365,260 inhibited this effect [concentration inhibiting 50% of maximal response (IC50), 2 x 10(-8) M], whereas the CCK-A antagonist L-364,718 (10(-8) M) and the tyrosine kinase inhibitor genistein (10(-4) M) had no effect. Histamine release was associated with a biphasic elevation of intracellular Ca2+. Gastrin stimulated HDC activity two- to threefold after 60 min of incubation (EC50, 10(-10) M). Gastrin also increased DNA synthesis in ECL cells, with an EC50 of 1.7 x 10(-12) M as measured by the incorporation of 5-bromo-2'-deoxyuridine (BrdU). Positive nuclear immunostaining increased two- to threefold in up to 20% of ECL cells af...Continue Reading

Citations

Sep 11, 2002·Annual Review of Physiology·Christian PrinzManfred Gratzl
Nov 16, 2002·Journal of Applied Physiology·Ian M P JosephDenise Kirschner
Jul 25, 2000·American Journal of Physiology. Cell Physiology·H L WaldumA K Sandvik
Jan 15, 2002·American Journal of Physiology. Cell Physiology·Robert ZannerChristian Prinz
Jul 5, 2003·Biological & Pharmaceutical Bulletin·Ko HasebeKazuo Watanabe
Mar 27, 2020·Frontiers in Endocrinology·Qiang ZengDong-Yu Guo
Oct 29, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·C AthmannG Sachs

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