PMID: 9437736Jan 23, 1998Paper

Gastrin induces IP3 formation through phospholipase C gamma 1 and pp60c-src kinase

Peptides
R R Yassin, J T Abrams

Abstract

We have previously reported that gastrin induces a rapid and transient tyrosine phosphorylation of phospholipase C gamma 1 (PLC gamma 1) in association with inositol 1,4,5-trisphosphate (IP3) formation in rat colonic epithelial cells (34). In this study, we demonstrate that gastrin regulates IP3 formation mainly through PLC gamma 1 isozyme. Immunoblotting analysis revealed the expression of PLC beta 3 and -gamma 1, but not PLC beta 1, -beta 2, or -beta 4 in the rat colonic epitheliums. To explore what PLC isozyme(s) modulates gastrin effect on IP3, immunoneutralizing antibody to PLC beta 1, -beta 3, or -gamma 1 was introduced into the colonic cells using a lipid carrier. The gastrin-stimulated increase in IP3 concentration was specifically prevented by anti-PLC gamma 1 but not by anti-PLC beta 1 or -beta 3 antibody. Immunoprecipitation assays have also revealed that gastrin promoted an increase in tyrosine phosphorylation and co-precipitation of a 60 kDa src kinase with PLC gamma 1. Administration of antibody specific to pp60c-src into the colonic cells prevented the gastrin-stimulated increases in IP3. Tyrosine phosphorylation of PLC gamma 1 may be a major mechanism through which gastrin regulates IP3 level in the colonic cell...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S A WankT Slattery
Apr 25, 1992·Proceedings of the National Academy of Sciences of the United States of America·A S KopinM Beinborn
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S A WankA de Weerth
Apr 1, 1990·Gastroenterology·A N Charney, H M Dansky
Jan 1, 1990·Gastroenterology·B RybergH Mattsson
Oct 1, 1989·Trends in Pharmacological Sciences·R T JensenJ D Gardner
Nov 1, 1995·The Biochemical Journal·R R Yassin, K M Little
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·G S Baldwin
Aug 30, 1994·Biochemical and Biophysical Research Communications·T B Shea
Mar 1, 1993·Proceedings of the Society for Experimental Biology and Medicine·A P Majumdar, M Wahby

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Citations

Jul 29, 1999·Journal of Hepatology·L BaiocchiG Alpini
Mar 30, 2010·The Journal of Biological Chemistry·Kimberle A AgleMichael B Dwinell
Oct 7, 2006·Current Opinion in Gastroenterology·P SinghA Owlia
Feb 22, 2001·Annual Review of Physiology·E Rozengurt, J H Walsh
May 18, 1999·Biochemical and Biophysical Research Communications·E A HodsonJ S Lymn
Jul 9, 2011·Oral Oncology·Peili ChenF Gary Toback
Jul 4, 2006·Physiological Reviews·Marlène DufresneDaniel Fourmy
Oct 22, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·P SinghA Owlia
Aug 5, 2004·The Journal of Biological Chemistry·Barbara Olszewska-PazdrakMark R Hellmich
Jan 21, 2016·American Journal of Physiology. Endocrinology and Metabolism·Tianbing LiuPedro A Jose
Sep 25, 2021·Nature Chemical Biology·Xuefeng ZhangQiang Zhao

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