Gastrin induces multidrug resistance via the degradation of p27Kip1 in the gastric carcinoma cell line SGC7901

International Journal of Oncology
Kun ZhuangHanqing Guo

Abstract

Multidrug resistance (MDR) is one of the major reasons for the failure of chemotherapy-based gastric carcinoma (GC) treatments, hence, biologically based therapies are urgently needed. Gastrin (GAS), a key gastrointestinal (GI) hormone, was found to be involved in tumor formation, progression, and metastasis. In this study, quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical staining analysis revealed a high level of expression of GAS in drug-insensitive GC tissues (P<0.01) and similar results were revealed in GC cell lines SGC7901 and its multidrug-resistant variants SGC7901/VCR and SGC7901/ADR. We constructed a eukaryotic expression vector pCDNA3.1(+)/GAS for GAS overexpression and recombinant lentiviral vectors for specific siRNA (siGAS). Transfection of pCDNA3.1(+)/GAS increased (P<0.05) while transfection of siGAS (P<0.05) and co-treated with paclitaxel (TAX) and vincristine (VCR) combination (TAX-VCR) decreased (P<0.01) the cell viability of SGC7901, SGC7901/VCR and SGC7901/ADR. Apoptosis rates of SGC7901/VCR and SGC7901/ADR were reduced by pCDNA3.1(+)/GAS and increased by siGAS (P<0.05). The apoptosis rates of SGC7901/VCR, SGC7901/ADR and SGC7901 were all upregulated (P<0.01) when cells wer...Continue Reading

References

Jan 20, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R M ConwayP L Penfold
Nov 13, 1999·Nature Cell Biology·A C CarranoM Pagano
Feb 5, 2000·Neuropeptides·Z MeraliH Anisman
Aug 7, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Manthena V S VarmaRamesh Panchagnula
May 8, 2004·Developmental Cell·Keiko NakayamaKeiichi I Nakayama
Jun 17, 2005·Expert Opinion on Pharmacotherapy·Angelo De Milito, Stefano Fais
Oct 18, 2005·Molecular and Cellular Biology·Taichi HaraKeiichi I Nakayama
Nov 25, 2005·Current Drug Delivery·Hristos GlavinasBalázs Sarkadi
Jan 16, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Andrea VarroD Mark Pritchard
Feb 2, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Leena GandhiCharles M Rudin
Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Maria StendahlGöran Landberg
Apr 3, 2013·Scandinavian Journal of Gastroenterology·Jens Peter GoetzeJens Frederik Rehfeld
Sep 17, 2013·Journal of Cellular Biochemistry·Lin-Hai YanQiang Xiao
Oct 22, 2013·International Journal of Oncology·Nevyana R HristovaWilfried Roth
Sep 10, 2014·PLoS Biology·Catherine A Del VecchioPiyush B Gupta
May 16, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·J Dinesh KumarGraham J Dockray
Aug 19, 2015·The International Journal of Biochemistry & Cell Biology·Su Su Thae HnitQihan Dong
Sep 26, 2015·Metallomics : Integrated Biometal Science·Kathryn M MarshallOneel Patel
Nov 13, 2015·Current Cancer Drug Targets·Kazuma Masumoto, Masatoshi Kitagawa
Apr 22, 2016·World Journal of Gastroenterology : WJG·Ying-Lin NiuHui-Hong Zhai
Jun 22, 2016·Oncotarget·Katie A LloydD Mark Pritchard

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Related Papers

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Haitao GuanXijing Wang
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Yafang WangDaiming Fan
© 2022 Meta ULC. All rights reserved