Gastrodin Attenuates Bilateral Common Carotid Artery Occlusion-Induced Cognitive Deficits via Regulating Aβ-Related Proteins and Reducing Autophagy and Apoptosis in Rats

Frontiers in Pharmacology
Bo LiuJing-Shan Shi

Abstract

Gastrodin (GAS), an active constituent extracted from Gastrodia elata Blume, is used to treat ischemic stroke, epilepsy, dizziness, and dementia for centuries in China. This study examined its effects on vascular dementia (VD) and the underlying molecular mechanisms. VD was established by ligation of bilateral common carotid artery occlusion (BCCAO). A total of 7 days after BCCAO surgery, GAS (15, 30, and 60 mg/kg) was orally administered for 28 consecutive days to evaluate therapeutic effects. Cognitive function was tested by the Morris water maze. The neuronal morphological changes were examined via Hematoxylin-Eosin staining. Flow cytometry was used for evaluating apoptosis in the hippocampi. The target protein expression was examined by Western blot. The results showed that BCCAO induced cognitive impairment, hippocampus CA1 and CA3 pyramidal neuron damage, beta-amyloid (Aβ) deposition, excessive autophagy, and apoptosis. GAS treatment significantly improved BCCAO-induced cognitive deficits and hippocampus neuron damage. Molecular analysis revealed that GAS exerted the protective effect via reducing the levels of Aβ1-40/42, APP, and β-site APP-cleaving enzyme 1 expression, and increasing Aβ-related protein, a disintegrin an...Continue Reading

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Citations

Mar 7, 2019·The Journal of Pharmacology and Experimental Therapeutics·Fa-Ju ChenJing-Shan Shi
Nov 27, 2019·Journal of Cellular and Molecular Medicine·Zhuona XiXiaolan Zhong
May 30, 2020·Journal of Molecular Neuroscience : MN·Minzhen DengManting Huang
Oct 3, 2018·Journal of Molecular Neuroscience : MN·Lu-Yao AoYun-Man Li
Jul 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Haodong ZhuWanying Wu
Mar 25, 2020·Phytotherapy Research : PTR·Wenqiang ZhangYue Hou
May 28, 2019·Current Medicinal Chemistry·Ze WangJing-Shan Shi

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Methods Mentioned

BETA
Flow cytometry

Software Mentioned

Quantity One
SPSS
200
MT

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