Gastrodin improves preeclampsia-induced cell apoptosis by regulation of TLR4/NF-κB in rats

Food Science & Nutrition
Zhixiong MeiBenqi Teng

Abstract

To explain gastrodin improved cell apoptosis induced by preeclampsia in vivo and in vitro study. The PE and normal rats were injected with normal saline (Model), low-dose gastrodin (Gas-L), medium-dose gastrodin (Gas-M), and high-dose gastrodin (Gas-H) groups at 50, 100, or 200 mg/kg per day. The rat blood pressure and 24-hr urine protein level were measured at pregnant days 10, 16, and 20. Evaluating pathology by H&E staining, the cell apoptosis by TUNEL, and MyD88 and NF-κB (p65) proteins by IHC assay using H/R to simulate PE cell model. Measuring cell proliferation, apoptosis, and MyD88 and NF-κB (p65) protein expression by MTT, flow cytometry, and WB assay. The SBP, DBP, and 24-hr urine protein levels were significantly different in PE rats (p < .05). The SBP, DBP, and 24-hr urine protein levels were significantly improved (p < .05) in vivo and in vitro. The positive apoptosis cells and apoptosis rate were significantly increased with MyD88 and NF-κB (p65) proteins upregulation (p < .05). The positive apoptosis cells and apoptosis rate were significantly decreased with MyD88 and NF-κB (p65) proteins depressing in gastrodin-treated groups with dose-dependent (p < .05). Gastrodin improves PE-induced cell apoptosis and patholo...Continue Reading

References

Mar 26, 2005·Archives of Gynecology and Obstetrics·Lina Fernández CeladillaManuel Muñoz Rodríguez
Apr 4, 2006·American Journal of Obstetrics and Gynecology·Berthold HuppertzJohn C P Kingdom
Oct 3, 2015·American Journal of Obstetrics and Gynecology·Susan J Fisher
Apr 21, 2016·Clinical Journal of the American Society of Nephrology : CJASN·Elizabeth PhippsBelinda Jim
May 24, 2016·Cardiovascular Journal of Africa·P Gathiram, J Moodley
Jul 31, 2016·Acta biochimica Polonica·Fangtao DuYenze Diao
Jan 6, 2017·Clinical Reviews in Allergy & Immunology·Jaume Alijotas-ReigJosep Maria Gris
Apr 10, 2017·Immunologic Research·Luiza Oliveira PerucciLirlândia Pires Sousa
Feb 2, 2019·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Sana ShahidGhulam Mustafa Khan
Feb 6, 2019·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Antonio TravaglinoMaria D'Armiento
Feb 21, 2019·International Journal of Immunogenetics·Valéria de Lima KaminskiJosé Artur Bogo Chies

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
PCR
flow cytometry

Software Mentioned

SCANSCOPE
ModFit LT

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao
B LuR Luo
Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association
Xi-Chang LiuGang Wu
© 2021 Meta ULC. All rights reserved