Gastrodin Protects Cardiomyocytes from Anoxia/Reoxygenation Injury by 14-3-3η

Oxidative Medicine and Cellular Longevity
Meifang ZhuBo Yi

Abstract

Gastrodin (GAS) is the major component isolated from the rhizome of the Chinese traditional medicinal herb "Tianma." Many clinical studies have found that GAS protects cardiomyocytes in cardiovascular diseases, although the effects and underlying mechanisms on cardiovascular anoxia/reoxygenation (A/R) injury remain unknown. This study is aimed at exploring the effect of gastrodin on cardiomyocytes in A/R injury. Our results suggested that the protective effect of GAS on cardiomyocytes is associated with upregulated 14-3-3η levels. Pretreatment with GAS could increase the cell viability and decrease the activities of creatine phosphokinase (CPK) and lactate dehydrogenase (LDH). GAS could also reduce reactive oxygen species (ROS) production, inhibit mitochondrial permeability transition pore (mPTP) opening, alter the maintenance of the mitochondrial membrane potential (∆Ψm), decrease the activation of caspase-3, and finally restrain cell apoptosis. Downregulating 14-3-3η levels by transfection with siRNA14-3-3η clearly attenuated the protective effect of GAS on cardiomyocytes in A/R injury.

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jul 9, 2004·Biochemical and Biophysical Research Communications·Narasimman GurusamyYoshifusa Aizawa
Mar 18, 2008·Biochemical Pharmacology·Rajarajan A ThandavarayanYoshifusa Aizawa
Apr 15, 2008·Biochemical and Biophysical Research Communications·Kazue GondaGoshi Shiota
Dec 9, 2008·The Biochemical Journal·Michael P Murphy
May 22, 2009·Journal of Cellular Biochemistry·Chia-Chun WangShun-Fen Tzeng
Jun 10, 2009·Journal of Medical Genetics·S GhavamiM Los
Jun 11, 2009·The Journal of Pharmacology and Experimental Therapeutics·Sabzali JavadovNelson Escobales
Aug 13, 2011·Molecular and Cellular Biochemistry·Chunming ShuQizhu Tang
Nov 15, 2011·Antioxidants & Redox Signaling·Ivana Novak
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Rajarajan A ThandavarayanKenichi Watanabe
Aug 28, 2012·International Journal of Molecular Medicine·Lihua ZhuHong Jiang
Jan 3, 2013·The Journal of Clinical Investigation·Derek J Hausenloy, Derek M Yellon
Jul 6, 2014·Physiological Reviews·Dmitry B ZorovSteven J Sollott
Jul 3, 2016·Molecular and Cellular Neurosciences·Divya Elizabeth MathewJoanne M Lewohl
Jul 6, 2016·Journal of Ethnopharmacology·Hong-Dan ZhanTing-Liang Jiang
Aug 17, 2016·Clinical and Translational Medicine·David KitzenbergLouise E Glover
Oct 23, 2016·Life Sciences·Fereshte BagheriHamid Khodayari
Oct 28, 2017·Frontiers in Molecular Neuroscience·Brett Cornell, Kazuhito Toyo-Oka
Feb 8, 2018·Oxidative Medicine and Cellular Longevity·José Carlos RiveraSylvain Chemtob

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
flow cytometry
protein assay
electrophoresis
transfection

Related Concepts

Related Feeds

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis