GATA-6 gene enhancer contains nested regulatory modules for primary myocardium and the embedded nascent atrioventricular conduction system

The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology
Richard F AdamoJohn B E Burch

Abstract

The cGATA-6 gene is flanked by an enhancer that selectively marks the atrioventricular conduction system (AVCS) in transgenic mice. This enhancer reads anterior/posterior and medial/lateral positional information very early in the cardiogenic program and remains active in progressively more restricted regions of primary myocardium leading up to the emergence of a histologically distinct AVCS. We undertook to parse this enhancer to resolve how the respective AVCS-specific transcription program is regulated at the molecular level. We determined that this AVCS enhancer includes a 102 bp module that is sufficient to restrict expression to primary nonchamber myocardium. This offers a novel tool to analyze the early molecular delineation of primary and chamber myocardium, which subsequently give rise to components of the central and peripheral conduction system, respectively. Furthermore, we show that this 102 bp module in turn contains a nested 47 bp core module that has the potential to direct expression specifically to the AVCS domain of primary myocardium, albeit with low efficiency. Accordingly, we show that a GATA site and a GC-rich site in the 102 bp region bolster the activity of the nested 47 bp AVCS core region even within ...Continue Reading

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K S ZaretC M DiPersio
Aug 1, 1995·Microscopy Research and Technique·J E Hall, R G Gourdie
Apr 18, 1998·Circulation Research·A F MoormanW H Lamers
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·R G GourdieT Mikawa
Apr 6, 1999·Trends in Cardiovascular Medicine·R G GourdieT Mikawa
Nov 26, 1999·Gene·G Suske
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Stacey RentschlerGlenn I Fishman
May 14, 2003·Journal of Cardiovascular Electrophysiology·Richard P KondoSylvia M Evans
Sep 25, 2003·Physiological Reviews·Antoon F M Moorman, Vincent M Christoffels
Mar 26, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Vincent M ChristoffelsMarina Campione
Apr 16, 2004·The Journal of Clinical Investigation·Patrick Y JaySeigo Izumo
May 26, 2004·Cardiovascular Research·Willem M H HoogaarsVincent M Christoffels

❮ Previous
Next ❯

Citations

Jun 19, 2013·Stem Cells and Development·Sherin I HashemWilliam C Claycomb
Jun 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jonathan T Butcher, Roger R Markwald
Sep 13, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Mathieu C RémondLeonard M Eisenberg
Apr 29, 2014·Nature Communications·Sonia StefanovicVincent M Christoffels
Oct 20, 2009·Journal of Molecular and Cellular Cardiology·Andrea BarbutiDario DiFrancesco
Mar 28, 2007·Heart Failure Clinics·Jau-Nian ChenJohn D Mably
Jun 2, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Wim T J AanhaanenVincent M Christoffels
Jan 22, 2005·Seminars in Cell & Developmental Biology·John B E Burch
Jan 21, 2016·Development·Jan Hendrik van Weerd, Vincent M Christoffels
Jul 2, 2015·Cellular and Molecular Life Sciences : CMLS·Sonia Stefanovic, Vincent M Christoffels
Feb 6, 2010·Circulation Research·Vincent M ChristoffelsAntoon F M Moorman
Sep 23, 2008·Journal of Cardiovascular Electrophysiology·Christopher RatnasamyRonald J Kanter
Jul 24, 2013·Molecular and Cellular Biochemistry·Sherin I Hashem, William C Claycomb
Oct 9, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Steven M White, William C Claycomb
Mar 30, 2021·Frontiers in Genetics·Xuefei YuanMichael D Wilson
Dec 15, 2004·Trends in Cardiovascular Medicine·Vincent M ChristoffelsAntoon F M Moorman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Conduction System

The cardiac conduction system is a specialized tract of myocardial cells responsible for maintaining normal cardiac rhythm. Discover the latest research on the cardiac conduction system here.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.