GATA factors and androgen receptor collaborate to transcriptionally activate the Rhox5 homeobox gene in Sertoli cells.

Molecular and Cellular Biology
Anjana BhardwajMiles F Wilkinson

Abstract

How Sertoli-specific expression is initiated is poorly understood. Here, we address this issue using the proximal promoter (Pp) from the Rhox5 homeobox gene. Its Sertoli cell-specific expression is achieved, in part, through a negative regulatory element that inhibits Pp transcription in non-Sertoli cell lines. Complementing this negative regulation is positive regulation conferred by four androgen-response elements (AREs) that interact with the androgen receptor (AR), a nuclear hormone receptor expressed at high levels in Sertoli cells. A third control mechanism is provided by a consensus GATA-binding site that is crucial for Pp transcription both in vitro and in vivo. Several lines of evidence suggested that GATA factors and AR act cooperatively to activate Pp transcription: (i) the GATA-binding site crucial for Pp transcription is in close proximity to two of the AREs, (ii) GATA and AR form a complex with the Pp in vitro, (iii) overexpression of GATA factors rescued expression from mutant Pp constructs harboring defective AREs, and (iv) incubation of a Sertoli cell line with testosterone triggered corecruitment of AR and GATA4 to the Pp. Collectively, our results suggest that the Rhox5 gene achieves Sertoli cell-specific tra...Continue Reading

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Citations

Oct 18, 2011·Asian Journal of Andrology·Qiao-Xia ZhangChawnshang Chang
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Anilkumar Bettegowda, Miles F Wilkinson
Jan 20, 2011·Molecular and Cellular Biology·James A MacleanMiles F Wilkinson
Apr 2, 2011·Reproductive Biology and Endocrinology : RB&E·Gargi BagchiDavid J Waxman
Nov 11, 2009·Molecular Endocrinology·Zhiying HuMiles F Wilkinson
Aug 23, 2012·PloS One·Subbarayalu PanneerdossManjeet K Rao
Jan 21, 2014·Molecular and Cellular Endocrinology·Lavinia VijaMarc Lombès
May 1, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·James A MacLean, Miles F Wilkinson
Apr 5, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Sergei G Tevosian
Nov 6, 2015·International Journal of Molecular Sciences·Wen LuoXiquan Zhang
Mar 23, 2011·The Journal of Urology·Ashley E RossEdward M Schaeffer
Jul 19, 2008·International Journal of Andrology·S ShankerM F Wilkinson
Mar 1, 2012·International Journal of Andrology·S O AbarikwuE O Farombi
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Mar 7, 2014·Molecular Reproduction and Development·Lihua YangYaoting Gui
Jun 19, 2013·Developmental Biology·Evgeni EfimenkoSergei G Tevosian
Jul 10, 2008·Nuclear Receptor Signaling·Frank ClaessensAnnemie Haelens
Oct 26, 2018·Human Reproduction·Kumar Mohanty SujitSingh Rajender
Oct 15, 2013·The Journal of Biological Chemistry·James A MacLeanMiles F Wilkinson
Aug 22, 2014·Development·Eric L BellLeonard Guarente

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