GATA3 Promotes the Neural Progenitor State but Not Neurogenesis in 3D Traumatic Injury Model of Primary Human Cortical Astrocytes

Frontiers in Cellular Neuroscience
Hilal CelikkayaCaghan Kizil

Abstract

Astrocytes are abundant cell types in the vertebrate central nervous system and can act as neural stem cells in specialized niches where they constitutively generate new neurons. Outside the stem cell niches, however, these glial cells are not neurogenic. Although injuries in the mammalian central nervous system lead to profound proliferation of astrocytes, which cluster at the lesion site to form a gliotic scar, neurogenesis does not take place. Therefore, a plausible regenerative therapeutic option is to coax the endogenous reactive astrocytes to a pre-neurogenic progenitor state and use them as an endogenous reservoir for repair. However, little is known on the mechanisms that promote the neural progenitor state after injuries in humans. Gata3 was previously found to be a mechanism that zebrafish brain uses to injury-dependent induction of neural progenitors. However, the effects of GATA3 in human astrocytes after injury are not known. Therefore, in this report, we investigated how overexpression of GATA3 in primary human astrocytes would affect the neurogenic potential before and after injury in 2D and 3D cultures. We found that primary human astrocytes are unable to induce GATA3 after injury. Lentivirus-mediated overexpres...Continue Reading

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Citations

Jan 17, 2020·Advanced Healthcare Materials·Sualyneth GalarzaShelly R Peyton
Mar 18, 2020·Frontiers in Cell and Developmental Biology·Violeta MashkaryanCaghan Kizil
Jan 16, 2021·Biomedicines·Laura MassozIsabelle Manfroid
Apr 30, 2021·STAR Protocols·Fabrizio PalumboEmre Yaksi
May 1, 2021·Micromachines·Tiffany CameronKaren C Cheung

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Datasets Mentioned

BETA
GSE116662
GSE117906

Methods Mentioned

BETA
transfection
Assay

Software Mentioned

GraphPad Prism
FIJI
JMP
Arivis
Arivis Vision
Vision 4D

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