GATA4 mediates activation of the B-type natriuretic peptide gene expression in response to hemodynamic stress

Endocrinology
M MarttilaH Ruskoaho

Abstract

To identify the mechanisms that couple hemodynamic stress to alterations in cardiac gene expression, DNA constructs containing the rat B-type natriuretic peptide (BNP) promoter were injected into the myocardium of rats, which underwent bilateral nephrectomy or were sham-operated. Ventricular BNP mRNA levels were induced about 4-fold; and the BNP reporter construct containing the proximal 2200 bp, 5-fold, in response to 1-d nephrectomy. Deletion of sequences between bp -2200 and -114 did not affect basal or inducible activity of the BNP promoter. An activator protein-1-like site and two tandem GATA elements are located within this 114-bp sequence. Both deletion and mutation of the AP-1-like motif decreased basal activity but did not abolish the response to nephrectomy. In contrast, mutation or deletion of -90 bp GATA-sites abrogated the response to hemodynamic stress. The importance of these GATA elements to BNP promoter activation was further confirmed by the corresponding 38-bp oligonucleotide conferring hemodynamic stress responsiveness to a minimal BNP promoter. In gel mobility shift assays, nephrectomy increased left ventricular BNP GATA4 binding activity significantly. In conclusion, GATA elements are necessary and suffici...Continue Reading

References

Aug 1, 1992·Cardiovascular Drugs and Therapy·A LochnerR Mouton
Jul 14, 1995·The Journal of Biological Chemistry·M Karin
Jan 1, 1993·Annual Review of Physiology·I Komuro, Y Yazaki
Jan 1, 1993·Annual Review of Physiology·K R ChienS M Evans
Sep 1, 1996·Molecular and Cellular Biology·D DurocherM Nemer
Jan 1, 1996·Cardiovascular Research·A J de BoldM L Kuroski de Bold
Mar 1, 1997·The Biochemical Journal·A M MurphyL Jones
Jan 1, 1997·Annual Review of Physiology·J Sadoshima, S Izumo
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·T C HerzigB E Markham
Oct 6, 1997·The EMBO Journal·D DurocherM Nemer
Jan 1, 1997·Methods in Cell Biology·A Kass-Eisler, L A Leinwand
May 16, 1998·The Journal of Biological Chemistry·M L Rosoff, N M Nathanson
Jun 11, 1998·Developmental Genetics·D Durocher, M Nemer
Apr 3, 1999·Circulation Research·E N Olson, J D Molkentin
Apr 3, 1999·Circulation Research·P H Sugden
Oct 21, 1999·The New England Journal of Medicine·J J Hunter, K R Chien
Dec 10, 1999·The Journal of Clinical Investigation·F Liang, D G Gardner
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·P ParadisM Nemer
Dec 7, 2000·Critical Reviews in Biomedical Engineering·C B HopkinsC Cook
Apr 1, 1997·Trends in Cardiovascular Medicine·T Evans

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Citations

Apr 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Anne AriesMona Nemer
Nov 2, 2005·Molecular and Cellular Biology·Jun WangMona Nemer
Jul 17, 2012·Journal of Cell Science·Akiko MammotoDonald E Ingber
Jun 12, 2010·Vascular Health and Risk Management·A PalazzuoliR Nuti
Jun 26, 2007·Molecular and Cellular Endocrinology·Theresa MajalahtiHeikki Ruskoaho
Mar 8, 2003·Hypertension·Michael H FreitagUNKNOWN Framingham Heart Study
May 31, 2003·Circulation Research·Hiroshi Akazawa, Issei Komuro
Jan 15, 2008·Journal of Hypertension·Zahid DhakamIan B Wilkinson
Oct 17, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Ken-ichi WatanabeYoshifusa Aizawa
Aug 17, 2019·IUBMB Life·Mika J Välimäki, Heikki J Ruskoaho
Apr 22, 2003·The Journal of Biological Chemistry·Sampsa PikkarainenHeikki Ruskoaho
Sep 13, 2002·The Journal of Biological Chemistry·Eva van RooijLeon J de Windt
Aug 3, 2002·The Journal of Biological Chemistry·Junliang PanQingyu Wu
Jun 16, 2009·Molecular and Cellular Endocrinology·T MajalahtiO Vuolteenaho

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