PMID: 9655631Jul 9, 1998Paper

Gating and permeability of ion channels produced by botulinum toxin types A and E in PC12 cell membranes

Toxicon : Official Journal of the International Society on Toxinology
Robert E Sheridan

Abstract

Botulinum neurotoxin (BoNT) is known to produce cationic channels in artificial bilayers. This study examined ion channels formed by BoNT in native membranes from cultured PC12 cells under conditions approximating those thought to occur during toxin internalization. Membrane patches were excised from PC12 cells using patch electrodes and exposed to symmetrical solutions containing either 200 mM CsCl, RbCl or KCl. The patch pipettes also contained 1-5 microg/ml BoNT buffered to pH 5.3 while the bath solutions were buffered to pH 7.0. In the presence of toxin, bursts of ion channel openings were observed. These toxin-induced channels were most active with a negative voltage applied to the same side as the toxin (cis). The increased activity at negative voltages was due to an increase in mean open time of e-fold per 120 mV and a decrease in mean closed time between bursts of e-fold per 110 mV. The shorter mean closed time within a burst was independent of membrane voltage. While BoNT-induced ion channels started as a single conductance level of 27 pS (KCl), 34 pS (RbCl) or 46 pS (CsCl) they typically increased in roughly equal steps to five or more times the original channel conductance. These higher conductance BoNT 'channels' op...Continue Reading

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Citations

Apr 20, 2004·Toxicology Letters·O RossettoC Montecucco
Nov 6, 2002·Trends in Biochemical Sciences·Kathryn TurtonK Ravi Acharya
May 8, 2008·Nature Clinical Practice. Urology·Michael B ChancellorK Roger Aoki
Dec 21, 2005·Acta Pharmacologica Sinica·Mu-feng Li, Yu-liang Shi
Dec 20, 2008·PLoS Pathogens·Audrey FischerMauricio Montal
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Audrey Fischer, Mauricio Montal
Aug 27, 2015·Biochimica Et Biophysica Acta·Marco PirazziniCesare Montecucco
Mar 17, 2007·Progress in Neurobiology·Yu-Liang Shi, Mu-Feng Li
Jun 30, 2006·Journal of Neurochemistry·Ornella RossettoCesare Montecucco
Jun 21, 2006·Neurotoxicity Research·A Fisher, M Montal
Feb 12, 2013·Toxicon : Official Journal of the International Society on Toxinology·Audrey Fischer, Mauricio Montal
Dec 4, 2012·Biochemical and Biophysical Research Communications·Marco PirazziniCesare Montecucco
Jul 1, 2014·Nature Reviews. Microbiology·Ornella RossettoCesare Montecucco
Oct 16, 1999·Journal of Molecular Biology·D B Lacy, R C Stevens

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