Gating charge differences between two voltage-gated K+ channels are due to the specific charge content of their respective S4 regions
Voltage-gated ion channels that differ in their primary amino acid sequence in the putative voltage sensor, the S4 region, show distinct voltage-sensing characteristics. In this study, we directly compared two voltage-gated K+ channels, the mammalian RCK1 with the Drosophila Shab11, and correlated the specific amino acid content of their respective S4 regions with the distinct voltage-sensing properties they exhibit. We find that specific differences in the charge content of the S4 region are sufficient to account for the distinct gating valence of each channel. However, differences in residues inside the S4 region are not sufficient to account for each channel's characteristic voltage range of activation.
Incremental reductions of positive charge within the S4 region of a voltage-gated K+ channel result in corresponding decreases in gating charge
Hydrophobic substitution mutations in the S4 sequence alter voltage-dependent gating in Shaker K+ channels
A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning
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Mutation of conserved negatively charged residues in the S2 and S3 transmembrane segments of a mammalian K+ channel selectively modulates channel gating
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Sodium channel activation gating is affected by substitutions of voltage sensor positive charges in all four domains
Glutamine substitution at alanine1649 in the S4-S5 cytoplasmic loop of domain 4 removes the voltage sensitivity of fast inactivation in the human heart sodium channel
Determinants of voltage-dependent gating and open-state stability in the S5 segment of Shaker potassium channels
Amino acid substitution within the S2 and S4 transmembrane segments in Shaker potassium channel modulates channel gating
The conformational analysis of a synthetic S4 peptide corresponding to a voltage-gated potassium ion channel protein
Differential effects of homologous S4 mutations in human skeletal muscle sodium channels on deactivation gating from open and inactivated states
Identification of a surface charged residue in the S3-S4 linker of the pacemaker (HCN) channel that influences activation gating.
Targeted replacement of KV1.5 in the mouse leads to loss of the 4-aminopyridine-sensitive component of I(K,slow) and resistance to drug-induced qt prolongation
Specific regions of heteromeric subunits involved in enhancement of G protein-gated K+ channel activity.
Specificity of Gbetagamma signaling to Kir3 channels depends on the helical domain of pertussis toxin-sensitive Galpha subunits
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