PMID: 2506548Sep 1, 1989Paper

Gating of single Shaker potassium channels in Drosophila muscle and in Xenopus oocytes injected with Shaker mRNA

Proceedings of the National Academy of Sciences of the United States of America
W N ZagottaR W Aldrich

Abstract

The voltage-dependent gating mechanism of single A-type potassium channels coded for by the Shaker locus of Drosophila was studied by single-channel recording. A-type channels expressed in Xenopus oocytes injected with Shaker B and Shaker D mRNA exhibited gating and voltage dependence that were qualitatively similar to those of the native Shaker A-types channels from embryonic myotubes. In all three channel types the molecular transition rates leading to the first opening were voltage-dependent, whereas all transitions after the first opening, including inactivation, were independent of voltage. While these channels exhibit some quantitative differences in their transition rates that account for the observed differences in macroscopic currents, in all three cases the voltage dependence of the macroscopic currents is determined by a voltage dependence in the time to first opening. This gating mechanism is similar to that of the vertebrate voltage-gated sodium channel and, together with the sequence similarities in the S4 region of the proteins, suggests a conserved mechanism for activation and inactivation.

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Citations

Aug 1, 1991·Journal of Bioenergetics and Biomembranes·M L GarciaG J Kaczorowski
Jun 19, 2009·European Biophysics Journal : EBJ·Krzysztof Małysiak, Zbigniew J Grzywna
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Dec 1, 1995·Biophysical Journal·E M OgielskaR W Aldrich
Mar 23, 2002·Free Radical Biology & Medicine·Jianguo ChenToshinori Hoshi
Sep 1, 2011·Nature Communications·Noah G ShuartWilliam N Zagotta
May 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·John R BankstonWilliam N Zagotta
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R J LeonardH A Lester
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K L ChoiG Yellen
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A E McEachernK Jarnagin
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·S E Gordon, W N Zagotta
Mar 28, 2008·The Journal of Biological Chemistry·Kimberley B CravenWilliam N Zagotta
Feb 1, 1994·The Journal of General Physiology·T HoshiR W Aldrich
Feb 1, 1994·The Journal of General Physiology·W N ZagottaR W Aldrich
Mar 1, 1995·The Journal of General Physiology·T Hoshi
May 6, 1999·The Journal of General Physiology·E R Sunderman, W N Zagotta
May 6, 1999·The Journal of General Physiology·E R Sunderman, W N Zagotta
Dec 2, 2004·The Journal of General Physiology·Kimberley B Craven, William N Zagotta
Jan 16, 2013·The Journal of General Physiology·Toshinori Hoshi, Clay M Armstrong
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Sep 28, 1990·Biochemical and Biophysical Research Communications·K McCormackB Rudy

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