Gating Protein Transport in Solid State Nanopores by Single Molecule Recognition

ACS Central Science
Gustav EmilssonAndreas B Dahlin

Abstract

Control of molecular translocation through nanoscale apertures is of great interest for DNA sequencing, biomolecular filters, and new platforms for single molecule analysis. However, methods for controlling the permeability of nanopores are very limited. Here, we show how nanopores functionalized with poly(ethylene glycol) brushes, which fully prevent protein translocation, can be reversibly gated to an "open" state by binding of single IgG antibodies that disrupt the macromolecular barrier. On the basis of surface plasmon resonance data we propose a two-state model describing the antibody-polymer interaction kinetics. Reversibly (weakly) bound antibodies decrease the protein exclusion height while irreversibly (strongly) bound antibodies do not. Our results are further supported by fluorescence readout from pore arrays and high-speed atomic force microscopy on single pores. This type of dynamic barrier control on the nanoscale provides new possibilities for biomolecular separation and analysis.

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Citations

Oct 12, 2019·Nanoscale·Olivia M EggenbergerMichael Mayer
Aug 17, 2020·Sensors·Lucile ReynaudArnaud Buhot
Oct 9, 2019·Nature Nanotechnology·Shuangshuang ZengZhen Zhang
Feb 6, 2021·Soft Matter·Yamila A Perez SirkinIgal Szleifer
Apr 15, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·John AnderssonAndreas Dahlin
Apr 30, 2021·Biosensors & Bioelectronics·Pedro V V RomanholoLivia F Sgobbi
Dec 8, 2020·ACS Applied Materials & Interfaces·Rachel A Lucas, Zuzanna S Siwy
Jan 6, 2021·The Journal of Physical Chemistry. B·C Pastorino, M Müller
Jan 8, 2022·Langmuir : the ACS Journal of Surfaces and Colloids·Daiki MurakamiYoshihisa Harada

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Methods Mentioned

BETA
surface plasmon resonance
atomic force microscopy
fluorescence microscopy
AFM

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