PMID: 7516427Jan 15, 1994Paper

Gating, selectivity and blockage of single channels activated by cyclic GMP in retinal rods of the tiger salamander

The Journal of Physiology
F SestiV Torre

Abstract

1. Patches in the inside-out configuration were excised from the membrane of outer and inner segments of the larval tiger salamander, Ambystoma tigrinum. The current flowing through single channels opened by cyclic GMP was studied with the voltage clamp technique. 2. Amplitude histograms of current recordings from patches containing only one flickering channel, excised from the inner segment and in the presence of 100 microM cyclic GMP, could be fitted by a theoretical scheme in which the single channel conductance was at least 55 pS at +40 mV and at least 45 pS at -40 mV. The mean open time was no longer than the time constant of our recording system, about 35 microseconds. Similar results were obtained by analysis of the amplitude histograms of patches from the outer segment containing many channels, and in the presence of 1-5 microM cyclic GMP. 3. In membrane patches excised from the outer segment, reducing the temperature from 24 to 8 degrees C did not reduce the flickering, but changed the amplitude histograms of current fluctuations activated by 1 microM cyclic GMP in a way consistent with a decrease of 50% in the single channel conductance and a decrease of 50% in the open probability. 4. In the presence of 1 microM cycl...Continue Reading

Citations

Jul 27, 1999·Neural Computation·P N Steinmetz, R L Winslow
Oct 22, 1993·Proceedings. Biological Sciences·M NizzariV Torre
Mar 18, 2009·The Journal of General Physiology·Monica MazzoliniVincent Torre
Jun 1, 1995·Current Opinion in Neurobiology·A L Zimmerman
Apr 24, 1999·Trends in Neurosciences·P FossierG Baux
Aug 7, 2021·Pflügers Archiv : European journal of physiology·Luisa Maria Rosaria NapolitanoArin Marchesi

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