Gating the selectivity filter in ClC chloride channels

Raimund DutzlerRoderick MacKinnon


ClC channels conduct chloride (Cl-) ions across cell membranes and thereby govern the electrical activity of muscle cells and certain neurons, the transport of fluid and electrolytes across epithelia, and the acidification of intracellular vesicles. The structural basis of ClC channel gating was studied. Crystal structures of wild-type and mutant Escherichia coli ClC channels bound to a monoclonal Fab fragment reveal three Cl- binding sites within the 15-angstrom neck of an hourglass-shaped pore. The Cl- binding site nearest the extracellular solution can be occupied either by a Cl- ion or by a glutamate carboxyl group. Mutations of this glutamate residue in Torpedo ray ClC channels alter gating in electrophysiological assays. These findings reveal a form of gating in which the glutamate carboxyl group closes the pore by mimicking a Cl- ion.


Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Dec 1, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C Miller
Jan 1, 1980·Annals of the New York Academy of Sciences·C Miller, M M White
Oct 1, 1996·The Journal of General Physiology·T Y Chen, C Miller
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·M MadukeJ A Mindell
Jun 29, 2001·The Journal of General Physiology·M F Chen, T Y Chen
Mar 28, 2002·Physiological Reviews·Thomas J JentschAnselm A Zdebik
May 17, 2002·Nature·Yu SunEric Gouaux
May 31, 2002·Nature·Youxing JiangRoderick MacKinnon
May 31, 2002·Nature·Youxing JiangRoderick MacKinnon
Oct 18, 2002·Nature·Ramkumar IyerChristopher Miller
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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