PMID: 9188562Jul 1, 1997Paper

GB virus B and hepatitis C virus NS3 serine proteases share substrate specificity

Journal of Virology
E ScarselliCinzia Traboni

Abstract

GB virus B (GBV-B) is a recently discovered virus responsible for hepatitis in tamarins (Saguinus species). GBV-B belongs to the Flaviviridae family and is closely related to the human pathogen hepatitis C virus (HCV). Nonstructural protein 3 (NS3) of HCV has been shown to encompass a serine protease domain required for viral maturation. GBV-B and HCV share only about 30% of the amino acid sequence within the NS3 protease domain. The catalytic triad is conserved, and the residue Phe-154, presumed to be a crucial amino acid for determining the S1 specificity pocket of the HCV NS3 protease, is also conserved. We have expressed a synthetic gene encoding the GBV-B NS3 protease domain in Escherichia coli and have characterized the purified recombinant protein for its activity on HCV substrates. We have shown that the NS3 region of the GBV-B genome actually encodes a serine protease that, despite the low sequence homology, shares substrate specificity with the HCV NS3 protease.

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Citations

Feb 22, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Stanley M LemonKui Li
Dec 21, 2007·Journal of Virology·David J WoollardEric J Gowans
Dec 30, 2014·Frontiers in Microbiology·Cordelia Manickam, R Keith Reeves
Jan 7, 2000·Journal of Virology·R RijnbrandS M Lemon
Aug 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Annette MartinStanley M Lemon
Jun 15, 2001·ILAR Journal·B BeamesR E Lanford
Sep 25, 2015·Journal of Virology·Caroline MarnataAnnette Martin
Feb 3, 2005·Lab Animal·Chandan GuhaJayanta Roy Chowdhury
Nov 13, 1999·Journal of Virology·A SbardellatiC Traboni
Aug 13, 1998·Journal of Medical Virology·L G BirkenmeyerI K Mushahwar
Sep 1, 2001·The Journal of Gene Medicine·L AurisicchioC Traboni
Nov 27, 1999·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·M RobaczewskaB Falkiewicz
Aug 26, 1998·Biochemistry·F MartinM Sollazzo

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