GBF-dependent family genes morphologically suppress the partially active Dictyostelium STATa strain.

Development Genes and Evolution
Nao ShimadaT Kawata

Abstract

Transcription factor Dd-STATa, a functional Dictyostelium homologue of metazoan signal transducers and activators of transcription proteins, is necessary for culmination during development. We have isolated more than 18 putative multicopy suppressors of Dd-STATa using genetic screening. One was hssA gene, whose expression is known to be G-box-binding-factor-dependent and which was specific to prestalk A (pstA) cells, where Dd-STATa is activated. Also, hssA mRNA was expressed in pstA cells in the Dd-STATa-null mutant. At least 40 hssA-related genes are present in the genome and constitute a multigene family. The tagged HssA protein was translated; hssA encodes an unusually high-glycine-serine-rich small protein (8.37 kDa), which has strong homology to previously reported cyclic-adenosine-monophosphate-inducible 2C and 7E proteins. Overexpression of hssA mRNA as well as frame-shifted versions of hssA RNA suppressed the phenotype of the partially active Dd-STATa strain, suggesting that translation is not necessary for suppression. Although overexpression of prespore-specific genes among the family did not suppress the parental phenotype, prestalk-specific family members did. Although overexpression of the hssA did not revert the e...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A Kuspa, W F Loomis
Sep 1, 1995·Developmental Biology·R Escalante, W F Loomis
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·T P SpannR H Gomer
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·G ShaulskyW F Loomis
Sep 12, 1997·Science·J E Darnell
Feb 28, 1998·Current Biology : CB·R R Kay
Jul 3, 1999·Trends in Genetics : TIG·G Prelich
Oct 20, 1999·Cellular and Molecular Life Sciences : CMLS·A L Mui
Feb 27, 2001·Nucleic Acids Research·F RiveroA A Noegel
Oct 31, 2001·Differentiation; Research in Biological Diversity·J H SeagerB D Hames
Apr 25, 2002·Nature Reviews. Genetics·Daniel St Johnston
Sep 10, 2003·Mechanisms of Development·Tetsuya MuramotoHideko Urushihara
Mar 12, 2004·The EMBO Journal·Kristine D GirardDouglas N Robinson
Sep 3, 2004·Development·Christina SchildeJeffrey G Williams
Oct 8, 2004·The International Journal of Developmental Biology·Nao ShimadaTakefumi Kawata
May 6, 2005·Nature·L EichingerA Kuspa
Aug 16, 2005·Molecular and Biochemical Parasitology·Kalpana LalRobert P Hirt
Dec 31, 2005·Nucleic Acids Research·Rex L ChisholmWarren A Kibbe
Jun 3, 2006·The International Journal of Developmental Biology·Ryota AoshimaTakefumi Kawata
Oct 20, 2006·Molecular Biology of the Cell·Edelyn OcttavianiDouglas N Robinson
Nov 7, 2006·Cellular Signalling·Hun Sik Kim, Myung-Shik Lee

❮ Previous
Next ❯

Citations

Oct 5, 2010·Development, Growth & Differentiation·Nao ShimadaTakefumi Kawata
Apr 13, 2013·BMC Developmental Biology·María Galardi-CastillaLeandro Sastre
Jul 28, 2010·Genetics·Jeffrey G Williams
Mar 11, 2016·Journal of Cell Science·Mariko Katoh-KurasawaGad Shaulsky

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.