GBS Mapping and Analysis of Genes Conserved between Gossypium tomentosum and Gossypium hirsutum Cotton Cultivars that Respond to Drought Stress at the Seedling Stage of the BC₂F₂ Generation

International Journal of Molecular Sciences
Richard Odongo MagwangaFang Liu

Abstract

Cotton production is on the decline due to ever-changing environmental conditions. Drought and salinity stress contribute to over 30% of total loss in cotton production, the situation has worsened more due to the narrow genetic base of the cultivated upland cotton. The genetic diversity of upland cotton has been eroded over the years due to intense selection and inbreeding. To break the bottleneck, the wild cotton progenitors offer unique traits which can be introgressed into the cultivated cotton, thereby improving their performance. In this research, we developed a BC₂F₂ population between wild male parent, G. tomentosum as the donor, known for its high tolerance to drought and the elite female parent, G. hirsutum as the recurrent parent, which is high yielding but sensitive to drought stress. The population was genotyped through the genotyping by sequencing (GBS) method, in which 10,888 single-nucleotide polymorphism (SNP) s were generated and used to construct a genetic map. The map spanned 4191.3 cM, with average marker distance of 0.3849 cM. The map size of the two sub genomes had a narrow range, 2149 cM and 2042.3 cM for At and Dt_sub genomes respectively. A total of 66,434 genes were mined, with 32,032 (48.2%) and 34,40...Continue Reading

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Citations

Apr 24, 2021·Scientific Reports·M S L R P MarasingheP C G Bandaranayake

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Methods Mentioned

BETA
genotyping
environmental stresses
environmental stress
DNA Assay
Assay
PCR
electrophoresis

Software Mentioned

MAS
GATK
Samtools
JoinMap
psRNATarget
GATK GBS pipeline
burrows wheeler aligner ( BWA )
custom
Blast2GO
perl script

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