GC-MS analysis of the ruminal metabolome response to thiamine supplementation during high grain feeding in dairy cows

Metabolomics : Official Journal of the Metabolomic Society
Fuguang XueBenhai Xiong

Abstract

Thiamine is known to attenuate high-concentrate diet induced subacute ruminal acidosis (SARA) in dairy cows, however, the underlying mechanisms remain unclear. The major objective of this study was to investigate the metabolic mechanisms of thiamine supplementation on high-concentrate diet induced SARA. Six multiparous, rumen-fistulated Holstein cows were used in a replicated 3 × 3 Latin square design. The treatments included a control diet (CON; 20% starch, dry matter basis), a SARA-inducing diet (SAID; 33.2% starch, dry matter basis) and SARA-inducing diet supplemented with 180 mg of thiamine/kg of dry matter intake (SAID + T). On d21 of each period, ruminal fluid samples were collected at 3 h post feeding, and GC/MS was used to analyze rumen fluid samples. PCA and OPLS-DA analysis demonstrated that the ruminal metabolite profile were different in three treatments. Compared with CON treatment, SAID feeding significantly decreased rumen pH, acetate, succinic acid, increased propionate, pyruvate, lactate, glycine and biogenic amines including spermidine and putrescine. Thiamine supplementation significantly decreased rumen content of propionate, pyruvate, lactate, glycine and spermidine; increase rumen pH, acetate and some medi...Continue Reading

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Citations

Sep 5, 2019·Asian-Australasian Journal of Animal Sciences·Hua ZhangLinshu Jiang
Mar 15, 2020·Animals : an Open Access Journal From MDPI·Maryam GholizadehLars Kaderali
Jun 3, 2020·Animals : an Open Access Journal From MDPI·Fuguang XueBenhai Xiong
Jun 19, 2021·Scientific Reports·Jessica Moraes MalheirosLuciana Correia de Almeida Regitano
Jun 11, 2019·Journal of Agricultural and Food Chemistry·Guoqin GeRuyan Hou

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Methods Mentioned

BETA
PCA

Software Mentioned

Chroma TOF
SAS
DA
R package
OPLS
SIMCA
Metabolome

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