GC-MS-Based Metabolomics to Reveal the Protective Effect of Gross Saponins of Tribulus terrestris Fruit against Ischemic Stroke in Rat

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Yang WangYajuan Xu

Abstract

Stroke is one of the most common neurological disorders and seriously threatens human life. Gross saponins of Tribulus terrestris fruit (GSTTF) are used for neuroprotective treatment on convalescents of ischemic stroke. However, the therapeutic effects and mechanisms have not yet well understood, especially from the metabolic perspective. In this study, the protective effect of GSTTF on ischemic stroke in a middle cerebral artery occlusion (MCAO) rat model was investigated by the GC-MS-based metabolomics approach. 2,3,5-triphenyltetrazolium chloride (TTC) staining of brain tissues showed that GSTTF significantly reduced the infarct area after MCAO surgery. Metabolomic profiling showed a series of metabolic perturbation occurs in ischemic stroke compared with sham group. GSTTF can reverse the MCAO-induced serum metabolic deviations by regulating multiple metabolic pathways including fatty acids metabolism, amino acids metabolism, and carbohydrates metabolism. The current study provided a useful approach for understanding the mechanism of MCAO-induced ischemic stroke and a reliable basis for evaluating the efficacy of GSTTF in the treatment of ischemic stroke.

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Citations

Jun 1, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Min WangRui Song
Jun 25, 2021·Frontiers in Cardiovascular Medicine·Yahong LiZhengfeng Xu
Jul 3, 2021·Reviews in the Neurosciences·Maria S ChumachenkoSergei V Fedorovich

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Software Mentioned

MetaboAnalyst
DA
XploreMET
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PLS
GraphPad
GraphPad Prism
SIMCA

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