GC-rich promoter elements maximally confers estrogen-induced transactivation of LRP16 gene through ERalpha/Sp1 interaction in MCF-7 cells

The Journal of Steroid Biochemistry and Molecular Biology
Weidong HanHai Jing Song

Abstract

LRP16 gene has been characterized as an estrogen-responsive gene. One 1/2ERE/GC-rich site was previously identified to be indispensable for -676/-214 (region A) fragment within LRP16 regulatory region to confer E2 action. Here, we report that -213/-24 fragment (region B) has higher E2-responsiveness than that of region A in MCF-7 cells, but not in HeLa cells. Deletion and mutation analyses of region B showed that multiple GC-sites are involved in the E2-stimulated response and one 30-bp fragment (-213 to -184 bp) is essential for conferring maximum E2-responsiveness. Results from the cotransfection assays containing Sp1-siRNA revealed that Sp1 is required for the basal transcription activity and E2-responsiveness of both regions A and B. Northern blot analysis demonstrated that inhibition of Sp1 in MCF-7 cells not only decreased the basal expression of LRP16, but markedly impaired its upregulation by E2. Results from gel mobility shift assays exhibited the direct binding of Sp1 protein to the 28-bp fragment (-211 to -184 bp), which was enhanced by the ERalpha titer. Moreover, the functional interaction of ERalpha and Sp1 proteins in the presence of E2 at the GC-rich sites in region B was confirmed by chromatin immunoprecipitati...Continue Reading

References

Jun 1, 1994·Endocrine Reviews·R T TurnerT C Spelsberg
Feb 7, 1997·Cell·R M ZwijsenR J Michalides
Jan 7, 1998·Molecular and Cellular Biology·T WatanabeM Muramatsu
Feb 22, 2001·Annual Review of Physiology·K Pettersson, J A Gustafsson
May 15, 2001·Endocrine-related Cancer·D C AllredS A Fuqua
Jun 1, 2002·Science·Donald P McDonnell, John D Norris
Feb 11, 2004·Endocrine Reviews·Carolyn L Smith, Bert W O'Malley
Sep 11, 2004·Molecular Endocrinology·Naoyuki FujitaPaul A Wade
Jan 6, 2006·Molecular Interventions·Julie M Hall, Donald P McDonnell
Mar 3, 2006·The Journal of Clinical Investigation·Bonnie J Deroo, Kenneth S Korach
May 9, 2006·Biochemical and Biophysical Research Communications·Jingang GuiFwu-Shan Sheu
May 9, 2006·Biochemical and Biophysical Research Communications·Kelly J HigginsStephen Safe

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Citations

Apr 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Junko TomikawaHiroko Tsukamura
May 12, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Lijuan ShaoKexing Fan
Jul 22, 2008·Medicinal Research Reviews·George G ChenGary Mk Tse
Jul 20, 2012·Breast Cancer Research : BCR·Tamara TanosCathrin Brisken
Nov 14, 2014·The FEBS Journal·Kate Beishline, Jane Azizkhan-Clifford

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