Feb 3, 2011

GCMS-based metabolomic study in mice with colitis induced by dextran sulfate sodium

Inflammatory Bowel Diseases
Yuuki ShiomiMasaru Yoshida

Abstract

Metabolomics provides data about all the metabolic processes of a cell or organism. So far, the changes that occur in the levels of metabolites during the development of colitis have not been fully elucidated. Here we examined the changes of metabolite levels in the serum and colon tissue of colitis mice using gas chromatography mass spectrometry (GC/MS) with the aim of achieving a detailed understanding of the pathogenesis of inflammatory bowel disease (IBD). To induce colitis, C57BL/6J mice were administered 3.0% dextran sulfate sodium (DSS) in their drinking water for 5 days and were subsequently given drinking water alone. A total of 77 and 92 metabolites were detected in serum and colon tissue, respectively, and among the metabolites the compositions of TCA cycle intermediates and amino acids changed depending on the degree of colitis. Then, partial least square discriminant analysis (PLS-DA), a multiple classification analysis, showed distinct clustering and clear separation of the groups according to the degree of colitis. Furthermore, PLS-DA loadings plots revealed that succinic acid, indole-3-acetic acid, glutamic acid, and glutamine were the main contributors to the separation of each stage of colitis. In addition, it...Continue Reading

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Mentioned in this Paper

Pathogenic Aspects
Taenia Coli
Pathogenesis
Papillon-Lefevre Disease
Amino Acids, I.V. solution additive
Gas Chromatography-Mass Spectrometry
Hyperkalemic Periodic Paralysis
Dextran Sulfate Sodium
Colitis
Therapeutic Agent

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