GC/MS-based urine metabolomics analysis of renal allograft recipients with acute rejection

Journal of Translational Medicine
Long ZhengDong Zhu

Abstract

Acute renal allograft rejection is a common complication after renal transplantation that often leads to chronic rejection and ultimate graft loss. While renal allograft biopsy remains the gold standard for diagnosis of acute rejection, the possibility of biopsy-associated complications cannot be overlooked. The development of noninvasive methods for accurate detection of acute renal allograft rejection is thus of significant clinical importance. Gas chromatography-mass spectrometry (GC/MS) was employed for analysis of urine metabolites in 15 renal allograft recipients with acute rejection and 15 stable renal transplant recipients. Partial least squares (PLS) regression and leave-one-out analyses were performed to ascertain whether the metabolites identified could be exploited to distinguish acute rejection from stable groups as well as their sensitivity and specificity. Overall, 14 metabolites were significantly altered in the acute rejection group (11 and 3 metabolites displayed higher and lower levels, respectively) relative to the stable transplant group. Data from PLS and leave-one-out analyses revealed that the differential metabolites identified not only distinguished acute rejection from stable transplant recipients but...Continue Reading

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Citations

Sep 3, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yan-Ni WangYing-Yong Zhao
Jun 8, 2021·Frontiers in Immunology·Xingqiang LaiZheng Jenny Zhang

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Methods Mentioned

BETA
biopsy
biopsies
chemical exchange saturation transfer

Software Mentioned

VB
SPSS
AMDIS

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