GC/MS detection of central nervous tissue as specified BSE risk material in meat products and meat and bone meals: thermal stability of markers in comparison with immunochemistry and RT-PCR

Analytical and Bioanalytical Chemistry
E LückerAndreas Hensel

Abstract

Methods for the detection of central nervous tissue (CNT) are urgently needed in food control as a means for controlling strict adherence to both food labeling and banning of specified BSE risk material. Here, we report data on heat stability of the CNT markers neuron-specific enolase (NSE) in western blotting, glial fibrillary acidic protein (GFAP) in an enzyme linked immunoassay, mRNA(GFAP) in a real-time PCR assay, and several fatty acids (C22:6, C24:0-OH, C24:1ω9/ω7, C24:1ω9-OH/ω7-OH, and C24:0) in gas chromatography mass spectrometry (GC/MS). The sample matrix, a standard material of emulsion-type sausage with varied contents of CNT (brain), was heat-treated in three studies: (1) routine meat technological heat treatment with low (85 °C, 30 min), medium (115 °C, 30 min), and high (133 °C, 30 min, 3 bar) heating of 72 anonymous samples from a blind trial; (2) heat treatment under experimental conditions (100, 110, …, 200 °C, 45 min); and (3) fractionized heating of central nervous system (up to three times) under moderate routine technological conditions (85, 100, and 115 °C, 30 min). The markers of the immunochemical methods showed a low GFAP or very low NSE temperature stability at medium and high temperature conditions. ...Continue Reading

References

Apr 6, 1996·Lancet·R G WillP G Smith
Mar 4, 2000·Journal of Food Protection·E H LückerM Bülte
Jun 29, 2004·Analytical and Bioanalytical Chemistry·Wolfgang BiedermannAndreas Hensel
Feb 9, 2005·Analytical and Bioanalytical Chemistry·Ernst LückerAndreas Hensel
Jul 21, 2005·Environmental Biosafety Research·Torsten BauerChristian Hertel
Oct 16, 2007·Journal of Chromatography. a·Maria GriessbachErnst Lücker
Jul 22, 2008·Journal of Veterinary Internal Medicine·B GlanemannM Bülte

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