GCN5, a yeast transcriptional coactivator, induces chromatin reconfiguration of HIS3 promoter in vivo

Biochemical and Biophysical Research Communications
P FileticiP Ballario

Abstract

Gcn5p, the nuclear histone acetyltransferase (HAT A), is a component of the multiprotein adaptor complex, ADA. Its role as a transcriptional coactivator is required for full induction of most of the genes regulated by GCN4. In this study we present experimental evidence demonstrating that, during gene activation, the nuclease sensitive region of HIS3 promoter, harbouring the poly (dA:dT) and the GCN4 binding site, is invaded by nucleosomes in a gcn5 disrupted strain. These data demonstrate, for the first time, that Gcn5p affects directly the chromatin organization of a chromosomal gene during its transcriptional activation.

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Citations

Oct 2, 2012·BMC Genomics·Paul Gagniuc, Constantin Ionescu-Tirgoviste
Dec 24, 2011·Acta Biochimica Et Biophysica Sinica·Wei-Jian SunZhi-Qiang Zheng
Sep 15, 2004·Journal of Molecular Biology·Cindy L White, Karolin Luger
Mar 26, 2002·FEBS Letters·Lei Zeng, Ming Ming Zhou
Nov 3, 2005·Journal of Cellular Biochemistry·Cong PengGui Yuan Li
Sep 14, 2010·Molecular and Cellular Biochemistry·Li LiChuan Feng
Aug 13, 2020·Metabolites·Hongting TangJay D Keasling

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