Abstract
We have previously shown that the expression of glucosylceramide synthase (GCS) gene in drug-resistant K562/AO2 human leukemia cell was higher than that in drug-sensitive K562 cell, and the sensitivity to adriamycin of K562/AO2 cell was enhanced by inhibiting GCS. It is concluded that the overexpression of GCS gene is one of the reasons which lead to multidrug resistance (MDR) of leukemia cell. Meanwhile, we also found that higher expression of Bcl-2 gene and protein were exhibited in K562/AO2 cell compared with K562 cell. Basing on this, we hypothesized that the high expression of GCS gene which results in MDR of leukemia cell is correlated with Bcl-2 signal transduction. In order to validate the hypothesis, the inhibition of GCS gene in K562/AO2 cell was observed by using chemical suppressor PPMP and siRNA targeted at GCS, and applying RT-PCR and flow cytometry, the expression levels of apoptosis-related gene Bcl-2 and Bax were analyzed before and after inhibiting GCS gene in K562/AO2 cell. The results demonstrated that the gene and protein of Bcl-2 in K562/AO2 cell were both down-regulated significantly after GCS gene being inhibited; however, the Bax mRNA expression had no apparent change in different groups. This suggested...Continue Reading
References
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K H CowanC E Myers
Aug 27, 1993·Cell·Z N OltvaiS J Korsmeyer
Mar 7, 1996·Nature·M VerheijR N Kolesnick
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·J ZhangY A Hannun
Aug 9, 1996·The Journal of Biological Chemistry·Y LavieM C Cabot
Dec 13, 1996·Science·Y A Hannun
Jan 17, 1997·The Journal of Biological Chemistry·Y LavieM C Cabot
Jan 23, 1997·Nature·A J MinnC B Thompson
Apr 29, 1998·Annual Review of Physiology·R N Kolesnick, M Krönke
Dec 29, 1998·The Journal of Biological Chemistry·D L MarksR E Pagano
Feb 26, 1999·Biochemical Pharmacology·N S Radin
Jul 27, 1999·Cancer·A LucciM C Cabot
Oct 3, 1999·British Journal of Cancer·K M NicholsonJ R Warr
Nov 26, 1999·Leukemia Research·N R WallA M Al-Katib
Nov 27, 1999·Leukemia Research·C PepperP Bentley
Mar 4, 2000·The Journal of Biological Chemistry·Y Y LiuM C Cabot
May 12, 2000·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·B YueZ Xiang
Jun 22, 2000·International Journal of Cancer. Journal International Du Cancer·J W KokM Müller
Dec 7, 2000·Journal of the National Cancer Institute·B J MaurerC P Reynolds
May 12, 2004·Life Sciences·C CarrionJ C Domingo
Jun 19, 2004·Leukemia Research·William R FriedenbergJohn C Reed
Sep 3, 2004·Cancer Research·Yoshikazu UchidaToshiro Okazaki
Mar 1, 2006·Molecular Cancer Therapeutics·David E ModrakDavid M Goldenberg
Aug 22, 2007·Leukemia Research·Ping XieKe-Ming Xie
Mar 26, 2008·Blood·John C Reed
Citations
Mar 8, 2011·International Journal of Hematology·Yang-Yang ZhangPing Xie
Dec 5, 2013·PloS One·Yan XuJun Y Fan
Oct 5, 2014·International Journal of Hematology·Qian WangPing Xie
Jul 14, 2011·Leukemia & Lymphoma·Rebecca J WattersThomas P Loughran
Nov 28, 2012·Leukemia & Lymphoma·Rebecca J WattersXin Liu
Mar 12, 2013·Progress in Lipid Research·Richard Jennemann, Hermann-Josef Gröne
Mar 20, 2012·Journal of Experimental & Clinical Cancer Research : CR·Min SongGuanrui Yang
Oct 18, 2012·Cancer Chemotherapy and Pharmacology·Melis Kartal YandımYusuf Baran
Apr 2, 2020·The Journal of Biological Chemistry·Eduardo J SalustianoJosé O Previato
Sep 5, 2020·Molecular Biology Reports·Sweta GhoshSubrata Majumdar
Jun 13, 2015·Molecular Cancer Therapeutics·Jong-Lyel RohJi Won Kim
Jun 4, 2021·Glycobiology·Safoura RezaJarosław Suchański