GD3-7-aldehyde is an apoptosis inducer and interacts with adenine nucleotide translocase

Biochemical and Biophysical Research Communications
Catherine BrennerRoger Sandhoff

Abstract

We prepared GD3-7-aldehyde (GD3-7) and determined its apoptotic potential. GD3-7 proved to be more efficient to induce pro-apoptotic mitochondrial alterations than GD3 when tested on mouse liver mitochondria. GD3-7-induced mitochondrial swelling and depolarization was blocked by cyclosporin A (CsA) supporting a critical role of the permeability transition pore complex (PTPC) during GD3-7-mediated apoptosis. In contrast to GD3, GD3-7 was able to induce channel formation in proteoliposomes containing adenine nucleotide translocase (ANT). This suggests that ANT is the molecular target of GD3-7. Using a specific antiserum, GD3-7 was detected in the lipid extract of the myeloid tumor cell line HL-60 after apoptosis induction, but not in living cells. Therefore, GD3-7 might be a novel mediator of PTPC-dependent apoptosis in cancer cells.

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Citations

May 23, 2012·Trends in Endocrinology and Metabolism : TEM·Mangesh PagadalaJohn P Kirwan
Jul 6, 2010·Mitochondrion·Lucia BiasuttoJiri Neuzil
Feb 1, 2015·Apoptosis : an International Journal on Programmed Cell Death·Carmen Garcia-RuizJosé C Fernández-Checa
Oct 30, 2020·Frontiers in Neuroscience·Simonetta SipioneVaibhavi Kadam
Jul 7, 2018·Chemical Reviews·Carmanah D HunterChristopher W Cairo

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