GdCl3 suppresses the malignant potential of hepatocellular carcinoma by inhibiting the expression of CD206 in tumor‑associated macrophages

Oncology Reports
Fangyu ZhuFang Luo

Abstract

In the present study, we aimed to ascertain whether there is a correlation between CD206 expression in tumor associated-macrophages (TAMs) and the prognosis of primary hepatocellular carcinomas (HCC) and we investigated the effect of GdCl3 on HCC. The expression of CD206 in HCC tumor tissues and peri-carcinoma tissues was measured using an array for liver tissues. The effects of GdCl3 on CD206 expression were examined in stimulated RAW264.7 cells. Target gene expression was evaluated by RT-PCR, western blotting and immunohistochemistry. The transwell system was used to assess the invasiveness of HCC cells. Finally, we established a mouse model for HCC using N-nitrosodiethylamine (DEN) to determine the effect of GdCl3 on HCC. Liver tissue array analysis revealed that CD206 was highly expressed in the HCC tissues compared to the level in peri-carcinoma tissue. We found that GdCl3 suppressed the expression of CD206 in the M2 macrophage phenotype of stimulated RAW264.7 cells with an IC10 value of 0.07 µg/µl. In addition, GdCl3 also induced cell apoptosis in the RAW264.7 cells. Addition of GdCl3 into the culture medium of RAW264.7 cells markedly reduced the invasive ability of Hepa1-6 cells compared to the control cells. Accordingly...Continue Reading

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Citations

May 8, 2018·The Journal of Pathology·Elizabeth Ann L EnningaSvetomir N Markovic
Mar 7, 2018·Journal of Experimental & Clinical Cancer Research : CR·Jian-Feng LiuZhi-Jun Sun
Nov 7, 2019·Histochemistry and Cell Biology·Steven A BloomerKevin C Kregel
Nov 5, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tatiana A KorolenkoVaclav Vetvicka

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