Gd(III)-Gd(III) distance measurements with chirp pump pulses

Journal of Magnetic Resonance
Andrin DollGunnar Jeschke

Abstract

The broad EPR spectrum of Gd(III) spin labels restricts the dipolar modulation depth in distance measurements between Gd(III) pairs to a few percent. To overcome this limitation, frequency-swept chirp pulses are utilized as pump pulses in the DEER experiment. Using a model system with 3.4 nm Gd-Gd distance, application of one single chirp pump pulse at Q-band frequencies leads to modulation depths beyond 10%. However, the larger modulation depth is counteracted by a reduction of the absolute echo intensity due to the pump pulse. As supported by spin dynamics simulations, this effect is primarily driven by signal loss to double-quantum coherence and specific to the Gd(III) high spin state of S=7/2. In order to balance modulation depth and echo intensity for optimum sensitivity, a simple experimental procedure is proposed. An additional improvement by 25% in DEER sensitivity is achieved with two consecutive chirp pump pulses. These pulses pump the Gd(III) spectrum symmetrically around the observation position, therefore mutually compensating for dynamical Bloch-Siegert phase shifts at the observer spins. The improved sensitivity of the DEER data with modulation depths on the order of 20% is due to mitigation of the echo reduction...Continue Reading

References

Dec 1, 1985·Physical Review A: General Physics·J BaumA Pines
Dec 13, 2001·Journal of Magnetic Resonance·M Garwood, L DelaBarre
Apr 12, 2002·Journal of Magnetic Resonance·Kristin E CanoA J Shaka
Oct 20, 2006·Angewandte Chemie·Adelheid GodtGunnar Jeschke
Mar 31, 2007·Journal of Magnetic Resonance·Michael K Bowman, Alexander G Maryasov
Jul 2, 2010·Journal of the American Chemical Society·Gunnar JeschkeAdelheid Godt
May 10, 2011·Physical Chemistry Chemical Physics : PCCP·Michal Gordon-GrossmanDaniella Goldfarb
Jun 15, 2011·Journal of the American Chemical Society·Hiromasa YagiGottfried Otting
Oct 8, 2011·Journal of Magnetic Resonance·Yuki AsadaHiroyuki Mino
Mar 1, 2012·Physical Chemistry Chemical Physics : PCCP·Ilia KaminkerDaniella Goldfarb
May 15, 2012·Journal of Magnetic Resonance·Philipp E SpindlerThomas F Prisner
Jun 30, 2012·Physical Chemistry Chemical Physics : PCCP·Maxim YulikovGunnar Jeschke
Jul 4, 2012·Physical Chemistry Chemical Physics : PCCP·Yevhen PolyhachGunnar Jeschke
Feb 5, 2013·The Journal of Physical Chemistry. B·Petra LuedersMaxim Yulikov
Feb 26, 2013·Journal of Magnetic Resonance·Andrin DollGunnar Jeschke
Feb 28, 2013·The Journal of Physical Chemistry. B·Luca GarbuioMaxim Yulikov
Jun 5, 2013·Physical Chemistry Chemical Physics : PCCP·Devin T EdwardsMark S Sherwin
Jan 17, 2014·Physical Chemistry Chemical Physics : PCCP·Daniella Goldfarb
Jul 27, 2014·Journal of Magnetic Resonance·Andrin Doll, Gunnar Jeschke
Oct 18, 2014·Journal of the American Chemical Society·Mian QiMalte Drescher
Dec 17, 2014·Journal of Magnetic Resonance·Philipp SchöpsThomas F Prisner
Feb 24, 2015·Physical Chemistry Chemical Physics : PCCP·Andrin DollGunnar Jeschke
May 6, 2015·The Journal of Physical Chemistry. B·Gunnar JeschkeAndrin Doll
Jun 20, 2015·Chemistry : a European Journal·Luca GarbuioGunnar Jeschke
Jun 26, 2015·Physical Chemistry Chemical Physics : PCCP·Arina DalaloyanDaniella Goldfarb
Aug 4, 2015·The Journal of Chemical Physics·Takuya F SegawaGunnar Jeschke
Aug 16, 2015·The Journal of Physical Chemistry Letters·Sahand RazzaghiMaxim Yulikov

❮ Previous
Next ❯

Citations

Jan 17, 2016·Journal of Magnetic Resonance·Stephan PribitzerGunnar Jeschke
Feb 24, 2016·The Journal of Organic Chemistry·Mian QiAdelheid Godt
May 11, 2016·The Journal of Physical Chemistry Letters·Christian HintzeMalte Drescher
Oct 21, 2016·Journal of Magnetic Resonance·Stephan PribitzerGunnar Jeschke
Oct 21, 2016·Journal of Magnetic Resonance·Katharina KellerMaxim Yulikov
Oct 28, 2016·Journal of Magnetic Resonance·Andrin DollGunnar Jeschke
Dec 16, 2016·Physical Chemistry Chemical Physics : PCCP·Andrin Doll, Gunnar Jeschke
Feb 14, 2017·Angewandte Chemie·Thomas WiegandAnja Böckmann
Jul 1, 2017·Physical Chemistry Chemical Physics : PCCP·Katharina KellerMaxim Yulikov
Jun 25, 2016·Physical Chemistry Chemical Physics : PCCP·Claudia E Tait, Stefan Stoll
Aug 6, 2016·Physical Chemistry Chemical Physics : PCCP·Andrin Doll, Gunnar Jeschke
Jul 27, 2017·Physical Chemistry Chemical Physics : PCCP·M J LawlessS Saxena
Apr 23, 2016·Physical Chemistry Chemical Physics : PCCP·Marie Ramirez CohenDaniella Goldfarb
May 25, 2016·Physical Chemistry Chemical Physics : PCCP·M P Donohue, V A Szalai
Jun 30, 2016·Physical Chemistry Chemical Physics : PCCP·A CollautoD Goldfarb
Oct 7, 2016·Physical Chemistry Chemical Physics : PCCP·Katharina KellerMaxim Yulikov
Nov 2, 2016·Physical Chemistry Chemical Physics : PCCP·D AkhmetzyanovS Un
Mar 13, 2019·Physical Chemistry Chemical Physics : PCCP·Angeliki GiannoulisDaniella Goldfarb
Dec 22, 2019·Proceedings of the National Academy of Sciences of the United States of America·Angeliki GiannoulisDaniella Goldfarb
Aug 14, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yin YangDaniella Goldfarb
Sep 25, 2020·Physical Chemistry Chemical Physics : PCCP·K KellerG Jeschke
Aug 3, 2017·The Journal of Chemical Physics·Nurit ManukovskyDaniella Goldfarb
Feb 1, 2017·Physical Chemistry Chemical Physics : PCCP·Jessica A ClaytonMark S Sherwin
Dec 16, 2020·Chembiochem : a European Journal of Chemical Biology·Adarshi P WelegedaraThomas Huber
Oct 1, 2018·Journal of Magnetic Resonance·Sergey MilikisiyantsAlex I Smirnov
Mar 10, 2021·The Journal of Chemical Physics·Jean-Baptiste VerstraeteMohammadali Foroozandeh
Feb 8, 2018·Journal of Magnetic Resonance·Alan BandVeronika A Szalai
Mar 28, 2021·Biophysical Journal·Thorsten BahrenbergDaniella Goldfarb
Oct 3, 2018·The Journal of Physical Chemistry Letters·Yin YangDaniella Goldfarb

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Physical Chemistry Chemical Physics : PCCP
Andrin DollGunnar Jeschke
Physical Chemistry Chemical Physics : PCCP
Daniella Goldfarb
Journal of Magnetic Resonance
Philipp SchöpsThomas F Prisner
Journal of the American Chemical Society
Arnold M RaitsimringDaniella Goldfarb
© 2021 Meta ULC. All rights reserved