GDNF-expressing STO feeder layer supports the long-term propagation of undifferentiated mouse spermatogonia with stem cell properties

Scientific Reports
Xiang WeiXin Wu


The development of a stem cell culture system would expedite our understanding of the biology of tissue regeneration. Spermatogonial stem cell (SSC) is the foundation for lifelong male spermatogenesis and the SSC culture has been optimized continuously in recent years. However, there have been many inconveniences to reconstruct SSC self-renewal and proliferation in vitro, such as the frequent refreshment of recombinant cytokines, including GDNF, the essential growth factor for SSC maintenance. In the present study, we observed that both STO and MEF cells, which were previously used as feeders for SSC growth, did not express GDNF, but a GDNF-expressing STO feeder could support undifferentiated mouse spermatogonia propagation in vitro for three months without the refreshment of recombinant growth factor GDNF. The cell morphology, growth rate and SSC-associated gene expression remained identical to the SSCs cultured using previous methods. The transplantation of SSCs growing on these GDNF-expressing STO feeders could generate extensive colonies of spermatogenesis in recipient testes, functionally validating the stemness of these cells. Collectively, our data indicated that the further modification of feeder cells might facilitate ...Continue Reading


Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·Ralph L Brinster, J W Zimmermann
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·M C HofmannJ L Millán
Feb 25, 1998·Biology of Reproduction·V BourdonG Pointis
Oct 27, 1998·Tissue & Cell·Makoto C NaganoRalph L Brinster
Apr 18, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi KubotaRalph L Brinster
Apr 30, 2008·Methods in Cell Biology·Hiroshi Kubota, Ralph L Brinster
Jul 1, 2009·BMC Developmental Biology·Ke ZhengPeijing Jeremy Wang
Nov 19, 2009·JAMA : the Journal of the American Medical Association·Hooman Sadri-ArdekaniAns M M van Pelt
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xin WuRalph L Brinster
Sep 17, 2010·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Apr 29, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hiroshi KubotaRalph L Brinster
Apr 27, 2012·Physiological Reviews·Jon M Oatley, Ralph L Brinster
Aug 11, 2012·Journal of Andrology·Dirk G de Rooij, Michael D Griswold
Oct 9, 2012·Cell Stem Cell·Mito Kanatsu-ShinoharaTakashi Shinohara
Feb 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Liang-Yu ChenEdward M Eddy
Apr 14, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Hong WangZuping He


Jul 4, 2018·Molecular Reproduction and Development·Beibei BieJin Sun
Apr 5, 2018·Biology of Reproduction·Hiroshi Kubota, Ralph L Brinster
Sep 24, 2019·Reproduction in Domestic Animals = Zuchthygiene·Huimin ZhaoShengsheng Lu

Methods Mentioned

confocal microscopy

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Related Papers

Zhonghua nan ke xue = National journal of andrology
Fujin ShenLinglong Wang
Proceedings of the National Academy of Sciences of the United States of America
Buom-Yong RyuRalph L Brinster
© 2021 Meta ULC. All rights reserved