GDP-mannose dehydrogenase is the key regulatory enzyme in alginate biosynthesis in Pseudomonas aeruginosa: evidence from metabolite studies

Microbiology
P J TatnellP Gacesa

Abstract

The Pseudomonas aeruginosa enzyme GDP-mannose dehydrogenase (GMD) is encoded by the algD gene, and previous genetic studies have indicated that it is a key regulatory and committal step in the biosynthesis of the polysaccharide alginate. In the present study the algD gene has been cloned into the broad-host-range expression vector pMMB66EH and GMD overexpressed in mucoid and genetically-related non-mucoid strains of P. aeruginosa. The metabolic approach of P. J. Tatnell, N. J. Russell & P. Gacesa (1993), J Gen Microbiol 139, 119-127, has been used to investigate the subsequent effect of GMD overexpression on the intracellular concentrations of the key metabolites GDP-mannose and GDP-mannuronate, which have been related to GMD activity and total alginate production. The overexpression of algD in mucoid and non-mucoid strains resulted in elevated GMD activities compared to wild-type strains; there was a concomitant reduction in GDP-mannose concentrations and greatly increased GDP-mannuronate concentrations. However, significantly, alginate biosynthesis was detected only in mucoid strains and GMD overexpression resulted in only a marginal increase in exopolysaccharide production. The GDP-mannuronate concentrations in mucoid strain...Continue Reading

Citations

Aug 17, 2006·Biotechnology Letters·Uwe Remminghorst, Bernd H A Rehm
Sep 17, 2013·Microbial Biotechnology·Iain D HayBernd H A Rehm
Oct 24, 2014·Frontiers in Chemistry·Christine Delbarre-LadratSylvia Colliec-Jouault
Jul 12, 2018·Molecular Microbiology·Yun Heacock-KangTung T Hoang
May 8, 2019·Journal of Proteome Research·Xia WuJames E Bruce

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