Gefitinib Inhibits Bleomycin-Induced Pulmonary Fibrosis via Alleviating the Oxidative Damage in Mice

Oxidative Medicine and Cellular Longevity
Li LiWeifeng Li

Abstract

Pulmonary fibrosis (PF) is a life-threatening interstitial lung disease. In this study, we tried to reveal the model of action between high-mobility group box 1 (HMGB1) and α-smooth muscle actin (α-SMA) and the protective role of gefitinib in pulmonary fibrosis induced by the administration of bleomycin aerosol in mice. For the mechanism study, lung tissues were harvested two weeks after modeling to detect the coexpression of HMGB1 and α-SMA by immunohistochemistry and immunofluorescence staining. Protein-DNA interactions were analyzed using a pulldown assay to study the relationship between HMGB1 and α-SMA. For the gefitinib treatment study, the mice were divided into three groups: phosphate-buffered saline (PBS) control group, PBS-treated PF group, and gefitinib-treated PF group. Gavage of gefitinib or PBS (20 mg/kg/day) was performed after bleomycin treatment for two weeks until the mice were sacrificed. Lung and blood samples were collected to assess the histological changes, oxidative stress, and expression of NOXs, HMGB1, EGFR, MAPKs, AP-1, and NF-κB to determine the curative effect and related molecular mechanisms. The results revealed the high coexpression of α-SMA and HMGB1 in some interstitial cells in the fibrotic lu...Continue Reading

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Citations

Jul 23, 2019·Journal of Animal Physiology and Animal Nutrition·Yulong HeHanchuan Dai
Oct 22, 2019·Cell Biology International·Karthika MuthuramalingamYoungmee Kim
Jul 17, 2019·BioMed Research International·Qinghua ZhangJunling Yang
Feb 25, 2020·Oxidative Medicine and Cellular Longevity·Yang ChenYanrong Lu
Jun 7, 2019·Antioxidants & Redox Signaling·Olga RafikovaRuslan Rafikov
Nov 25, 2020·Molecular Biology Reports·Yue FangPengxiu Cao
Jun 3, 2021·Pharmaceutics·Lívia A TavaresRicardo L C de Albuquerque-Júnior

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Datasets Mentioned

BETA
U63129
M57409

Methods Mentioned

BETA
Fluorescence
Pulldown
PCR
electrophoresis
MDA

Software Mentioned

GraphPad
GraphPad Prism
Leica Application Suite Advanced Fluorescence

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