Gel electrophoresis-based plant proteomics: Past, present, and future. Happy 10th anniversary Journal of Proteomics!

Journal of Proteomics
Jesus V Jorrín-NovoLuis E Rodríguez de Francisco

Abstract

In this century we have assisted at an unimaginable expansion of proteomics, with continuous innovations and optimizations in methods, techniques, protocols, equipment, and associated bioinformatics tools. We have moved forward very fast from first (gel electrophoresis based), to second (based on isotopic or isobaric labelling), to third (shotgun or gel-free, label-free), and to fourth (targeted, mass-western, or SRM/MRM) generation techniques. This evolution is clearly observed in the literature since 1994, when the term "proteome" was first coined, with plant proteomics progressing at a much lower speed than human and other model organisms. The question behind this review is: Is gel electrophoresis an obsolete technique? Is it still alive? The answer is that gel electrophoresis is still a valid technique, with its own particularities, strengths, and weaknesses, "irreplaceable" in top-down experiments directed at investigating protein species, loci and allelic variants, and isoforms, as well as in the post-translational modifications and interactions studies; it is an excellent complementary and alternative approach that could lead us to achieve a deeper visualization and knowledge of the cell proteome. The past, present, and ...Continue Reading

Citations

Feb 10, 2019·International Journal of Molecular Sciences·María-Dolores ReyJesús V Jorrín-Novo
Nov 2, 2019·International Journal of Molecular Sciences·Xinmin Ren, Xiangdong Li
Mar 21, 2020·BMC Bioinformatics·Qiaofeng OuBangshu Xiong
May 24, 2019·International Journal of Molecular Sciences·Setsuko Komatsu
Jan 9, 2021·BMC Genomics·Ricardo Nilo-PoyancoAndrea Miyasaka Almeida
Dec 5, 2020·International Journal of Molecular Sciences·Galina SmolikovaAndrej Frolov
Feb 24, 2021·Emerging Topics in Life Sciences·Amanda L Smythers, Leslie M Hicks
Mar 30, 2021·Frontiers in Plant Science·Kamil MikulášekZbyněk Zdráhal
Mar 27, 2021·Expert Review of Proteomics·J V Jorrin Novo
Jul 29, 2021·Journal of Agricultural and Food Chemistry·Mahya BahmaniMichelle L Colgrave
Jan 23, 2022·Journal of Fluorescence·Hassan Ali AbidTuck Wah Ng

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