Gel filtration analysis of vanadium in Ascidia nigra blood cell lysate

Journal of Cellular Physiology
K GilbertG C McLeod

Abstract

Fractions from a Sephadex gel filtration of homogenized Ascidia nigra blood cell lysate were analyzed for vanadium by atomic absorption spectroscopy. The results were unaffected by temperature from 4-21 degrees C, and by ionic strength in the range 0.09-1.0 M (NaCl). Appreciable loss of vanadium in the supernatant was experienced above pH 2.2. Experiments at pH 2.1 under anaerobic conditions show that the green chromogen and the vanadium-containing bands elute separately. Under these experimental conditions, the vanadium-containing species is of relatively low (congruent to 1,000) molecular weight.

References

Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R M Carlson
Apr 1, 1974·The Biological Bulletin·J H SwinehartN C Schroeder
Jun 1, 1954·Archives of Biochemistry and Biophysics·E BOERI, A EHRENBERG
Feb 1, 1963·Comparative Biochemistry and Physiology·L S CIERESZKOC A LANE

Citations

Nov 26, 1986·Biochemical and Biophysical Research Communications·H MichibataH Sakurai

Related Concepts

Molecular Sieve Chromatography
Ascidia
Vanadium

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