Gel-free/label-free proteomic, photosynthetic, and biochemical analysis of cowpea (Vigna unguiculata [L.] Walp.) resistance against Cowpea severe mosaic virus (CPSMV)

Journal of Proteomics
Anna L N VarelaJosé T A Oliveira

Abstract

Cowpea severe mosaic virus (CPSMV) causes significant losses in cowpea (Vigna unguiculata) production. In this present study biochemical, physiological, and proteomic analysis were done to identify pathways and defense proteins that are altered during the incompatible interaction between the cowpea genotype BRS-Marataoã and CPSMV. The leaf protein extracts from mock- (MI) and CPSMV-inoculated plantlets (V) were evaluated at 2 and 6days post-inoculation (DPI). Data support the assumptions that increases in biochemical (high hydrogen peroxide, antioxidant enzymes, and secondary compounds) and physiological responses (high photosynthesis index and chlorophyll content), confirmed by label-free comparative proteomic approach, in which quantitative changes in proteasome proteins, proteins related to photosynthesis, redox homeostasis, regulation factors/RNA processing proteins were observed may be implicated in the resistance of BRS-Marataoã to CPSMV. This pioneering study provides information for the selection of specific pathways and proteins, altered in this incompatible relationship, which could be chosen as targets for detailed studies to advance our understanding of the molecular, physiological, and biochemistry basis of the res...Continue Reading

References

Mar 9, 2000·Journal of Virology·C Reichel, R N Beachy
Jul 23, 2003·Nature Structural Biology·André Remy, Klaus Gerwert
Sep 1, 2005·Nature Reviews. Microbiology·Jennifer L M SoosaarSavithramma P Dinesh-Kumar
Oct 27, 2005·Molecular & Cellular Proteomics : MCP·Jesper V OlsenMatthias Mann
Sep 5, 2006·Plant Physiology·Daisuke IgarashiChieko Ohsumi
Jan 5, 2007·Photosynthesis Research·María Luisa Pérez-BuenoMatilde Barón
Apr 3, 2009·Journal of Proteome Research·Markus BroschJyoti Choudhary
Apr 8, 2009·Molecular Plant-microbe Interactions : MPMI·Melvin D Bolton
Jul 10, 2009·Analytical Chemistry·Ying ZhangLaurence Florens
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Phillip A HarriesRichard S Nelson
Oct 10, 2009·Plant Signaling & Behavior·Xiong-Yan Chen, Jae-Yean Kim
Jan 30, 2010·Advances in Virus Research·V Truniger, M A Aranda
Jul 16, 2011·Plant Signaling & Behavior·Changming Ye, Jeanmarie Verchot
Aug 2, 2011·Biochimica Et Biophysica Acta·Terry M BrickerJulian J Eaton-Rye
May 29, 2012·Frontiers in Plant Science·William Underwood
Aug 18, 2012·The New Phytologist·Ari SadanandomStuart Nelis
Jan 15, 2013·Journal of Proteomics·Setsuko KomatsuMinoru Nishimura
Feb 19, 2013·Trends in Plant Science·Elizabeth H NeilsonBirger Lindberg Møller
May 11, 2013·Genetics and Molecular Research : GMR·L S A GonçalvesV M Gomes
Oct 8, 2013·Plant Cell Reports·Qinyang YuWenli Li
Feb 28, 2014·Frontiers in Plant Science·Clemencia M RojasKirankumar S Mysore
Jun 19, 2014·Molecular Plant-microbe Interactions : MPMI·Muthukumar BalasubramaniamL Sue Loesch-Fries
Dec 3, 2014·Current Opinion in Plant Biology·Aline VoxeurRichard Sibout
Dec 22, 2014·Journal of Plant Research·Dhinesh KumarJae-Yean Kim
Jan 15, 2015·Molecular Plant-microbe Interactions : MPMI·Rosa Lozano-Durán, Silke Robatzek
Mar 12, 2015·Plant Signaling & Behavior·Vivek AmbasthaBudhi Sagar Tiwari
Jun 27, 2015·Viruses·Hélène Sanfaçon
Aug 13, 2015·Plant, Cell & Environment·Christine H Foyer, Graham Noctor
Nov 4, 2015·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob

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Citations

Jul 30, 2019·Theoretical and Experimental Plant Physiology·Pedro F N SouzaFabricio E L Carvalho

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