Gel mobility shift scanning of the acetate-inducible promoters from Neurospora crassa reveals a common co-inducible DNA-binding protein

Molecular & General Genetics : MGG
T MizoteI F Connerton

Abstract

The promoter regions of four acetate-inducible genes of Neurospora crassa, acu-3, acu-5, acu-8 and acu-9, have been sequenced. Using a scanning gel mobility shift assay particular DNA regions in each promoter have been shown specifically to bind partially purified protein extracted from acetate-induced mycelia. The protein-binding regions so defined have common sequence motifs, elements of which are similar to those required for acetate induction in aspergillus nidulans.

References

Feb 1, 1976·Journal of General Microbiology·S ArmittC F Roberts
Sep 1, 1991·Molecular & General Genetics : MGG·R A SandemanI F Connerton
Dec 1, 1989·Molecular and Cellular Biology·M E Katz, M J Hynes
Jan 1, 1987·Methods in Enzymology·L Hennighausen, H Lubon
Sep 1, 1986·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M J Hynes, M A Davis
Apr 1, 1966·The Biochemical Journal·H L Kornberg

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Citations

Nov 7, 1998·Biochimica Et Biophysica Acta·M BibbinsI F Connerton
Oct 20, 2005·Journal of Bioscience and Bioengineering·Y KashimaF Yanagida

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