Gelatin and galactomannan-based scaffolds: Characterization and potential for tissue engineering applications

Carbohydrate Polymers
Nataly M SiqueiraRosane M D Soares

Abstract

In this work, we produced gelatin films containing different concentrations of galactomannan by casting solutions. The films were crosslinked by immersion in 30mM solution of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC). The crosslinking of gelatin-containing films was confirmed by the reduction of free amine band intensity (3400-3200cm(-1)) in the GEL IR, as well as by the evaluation of its behavior when immersed in phosphate-buffer solution. The crosslinking of galactomannan film was confirmed by the formation of new ether bonds, as observed by increasing intensity of the band at 1148cm(-1), and the reduction of OH band intensity (3600-3200cm(-1)). The presence of galactomannan and the crosslinking mediated by EDC were responsible to improve elasticity in the gelatin-based films. The samples did not show cytotoxicity during 24h or 48h. In addition, rat mesenchymal stem cells adhered to the films regardless of galactomannan concentration. The results indicated that the gelatin/galactomannan films are potential biomaterials for use as scaffolds for tissue engineering.

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Citations

Sep 4, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Dibyajyoti BiswalKunal Pal
Feb 2, 2017·Journal of Materials Science. Materials in Medicine·Nicholas A TemofeewScott A Sell
May 3, 2019·Applied Biochemistry and Biotechnology·Kedong SongTianqing Liu
Nov 17, 2020·International Journal of Biological Macromolecules·Laís Ramos Monteiro LimaRegina Célia Monteiro de Paula
Dec 21, 2019·International Journal of Biological Macromolecules·Andressa Amado MartinMaria Rita Sierakowski

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