Gelatin based microfluidic devices for cell culture

Lab on a Chip
A Paguirigan, D J Beebe

Abstract

We have developed a technique for fabricating microfluidic devices from gelatin using a natural crosslinking process. Gelatin, crosslinked with the naturally occurring enzyme transglutaminase is molded to produce microchannels suitable for adherent cell culture and analysis. The autofluorescence of the material was shown to be minimal and within the range of typical background, ensuring utility with analyses using fluorescent dyes and labels would not be affected. Also, normal murine mammary epithelial cells were successfully cultured in the microchannels. The morphology of these adherent epithelial cells was shown to be significantly different for cells grown on rigid tissue culture plastic in either macro- or microscale cultures (even in the presence of a surface coating of gelatin) than those grown on the flexible crosslinked gelatin microchannels. Using these devices, the effects of both the extracellular matrix and soluble factors on cellular behavior and differentiation can be studied in microenvironments that more closely mimic the in vivo environment.

References

Aug 24, 2000·Journal of Agricultural and Food Chemistry·A MizunoM Motoki
Mar 14, 2002·Lasers in Surgery and Medicine·Gregory M PalmerNirmala Ramanujam
Jul 16, 2002·Annual Review of Biomedical Engineering·David J BeebeGlenn M Walker
Nov 12, 2002·Biomacromolecules·Vittorio CrescenziAnna Taglienti
Jul 8, 2003·The Journal of Pathology·Erik H J Danen, Arnoud Sonnenberg
Aug 19, 2003·Biomaterials·Heungsoo ShinAntonios G Mikos
Aug 19, 2003·Biomaterials·Ulrich HerselHorst Kessler
Nov 11, 2003·Biomacromolecules·Tianhong ChenGregory F Payne
Dec 3, 2003·The Journal of Pathology·Erik H J Danen, Arnoud Sonnenberg
Feb 27, 2004·Investigative Ophthalmology & Visual Science·Siegfried G PriglingerUlrich Welge-Lussen
Mar 25, 2004·Journal of Cellular Physiology·Francesco RossoAlfonso Barbarisi
Mar 31, 2004·Lab on a Chip·Glenn M WalkerDavid J Beebe
May 26, 2004·Lab on a Chip·Stephanie RatyMatthew B Wheeler
Jul 13, 2004·Biochimica Et Biophysica Acta·Michele A WozniakPatricia J Keely
Sep 14, 2004·Biomacromolecules·Sébastien GiraudierVéronique Larreta-Garde
Oct 19, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Emmett P BroderickAbhay S Pandit
Dec 14, 2004·Tissue Engineering·Kelly J ShieldsJennifer S Wayne
Jan 18, 2005·Seminars in Cancer Biology·Mark S Condon
Sep 22, 2005·Lab on a Chip·Hongmei YuDavid J Beebe
Sep 30, 2005·Nature·Javier Atencia, David J Beebe
Oct 20, 2005·Journal of Bioscience and Bioengineering·Akira ItoTakeshi Kobayashi

❮ Previous
Next ❯

Citations

Nov 28, 2012·Applied Microbiology and Biotechnology·Tobias HeckLinda Thöny-Meyer
May 27, 2008·Journal of Industrial Microbiology & Biotechnology·Shie-Jea LinWen-Shen Chu
May 28, 2013·Tissue Engineering. Part B, Reviews·Nihal E VranaAli Khademhosseini
Jul 19, 2008·Annual Review of Analytical Chemistry·Ivar Meyvantsson, David J Beebe
Dec 12, 2007·BMC Biotechnology·Thomas LiebmannHjalmar Brismar
Jan 5, 2007·Lab on a Chip·Michael W Toepke, David J Beebe
Jun 1, 2007·Lab on a Chip·Yibo LingAli Khademhosseini
Apr 25, 2008·Lab on a Chip·Ivar MeyvantssonDavid J Beebe
Sep 16, 2011·Lab on a Chip·Jarupat LuechaJozef L Kokini
Jan 22, 2013·BioNanoScience·Qiucen Zhang, Robert H Austin
Jul 18, 2015·International Journal of Molecular Sciences·Juan LiuArndt F Schilling
Jul 1, 2010·Expert Opinion on Drug Discovery·Cornelia CarstensHolger Becker
Oct 1, 2008·Expert Opinion on Drug Discovery·Yuan Wen, Shang-Tian Yang
Nov 13, 2015·Analytical Chemistry·Alexander K Price, Brian M Paegel
Oct 4, 2012·Trends in Neurosciences·Larry J Millet, Martha U Gillette
Oct 29, 2011·Trends in Cell Biology·Dongeun HuhDonald E Ingber
Mar 5, 2016·Macromolecular Bioscience·Emilia I WisotzkiMareike Zink
Jun 24, 2009·Biomaterials·Christopher MoraesCraig A Simmons
Dec 15, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Jiankang HeZhongmin Jin
Mar 26, 2014·Scientific Reports·S Cosson, M P Lutolf
Oct 1, 2010·Advanced Materials·Brandon V SlaughterNicholas A Peppas
Apr 11, 2009·Biotechnology and Bioengineering·Amy K ManocchiHyunmin Yi
Jun 23, 2007·Journal of Biomedical Materials Research. Part a·C W YungT A Barbari
Aug 27, 2010·Journal of Biomedical Materials Research. Part a·Chong Wing YungTimothy A Barbari
Jun 3, 2014·Macromolecular Materials and Engineering·Michael W ToepkeWilliam L Murphy
Apr 26, 2013·Biotechnology and Bioengineering·Cara Buchanan, Marissa Nichole Rylander
Mar 11, 2010·Advanced Materials·Peter DomachukDavid L Kaplan
Dec 16, 2008·IEEE Reviews in Biomedical Engineering·Jay W WarrickDavid J Beebe
Jun 13, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Qudus HamidWei Sun
Jun 7, 2014·Colloids and Surfaces. B, Biointerfaces·Yi-Long HanJin-Ye Wang
Apr 14, 2016·Bioanalysis·Chun-Wei ChiSihong Wang
Apr 4, 2015·Tissue Engineering. Part a·Muhammad SuhaeriKwideok Park

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.