Gelatin-Methacryloyl (GelMA) Hydrogels with Defined Degree of Functionalization as a Versatile Toolkit for 3D Cell Culture and Extrusion Bioprinting

Bioengineering
Iliyana PepelanovaAntonina Lavrentieva

Abstract

Gelatin-methacryloyl (GelMA) is a semi-synthetic hydrogel which consists of gelatin derivatized with methacrylamide and methacrylate groups. These hydrogels provide cells with an optimal biological environment (e.g., RGD motifs for adhesion) and can be quickly photo-crosslinked, which provides shape fidelity and stability at physiological temperature. In the present work, we demonstrated how GelMA hydrogels can be synthesized with a specific degree of functionalization (DoF) and adjusted to the intended application as a three-dimensional (3D) cell culture platform. The focus of this work lays on producing hydrogel scaffolds which provide a cell promoting microenvironment for human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) and are conductive to their adhesion, spreading, and proliferation. The control of mechanical GelMA properties by variation of concentration, DoF, and ultraviolet (UV) polymerization conditions is described. Moreover, hAD-MSC cell viability and morphology in GelMA of different stiffness was evaluated and compared. Polymerized hydrogels with and without cells could be digested in order to release encapsulated cells without loss of viability. We also demonstrated how hydrogel viscosity can be incr...Continue Reading

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Citations

Mar 6, 2019·Biofabrication·Qing GaoYong He
May 1, 2020·Advanced Healthcare Materials·Xiaolin CuiTim Woodfield
Oct 19, 2019·Journal of Biomedical Science·Hung-Jin HuangShan-Hui Hsu
Oct 2, 2019·Frontiers in Bioengineering and Biotechnology·Laura J BrayNathalie Bock
Jun 17, 2020·Macromolecular Bioscience·Antonina LavrentievaIliyana Pepelanova
Aug 15, 2018·Bioengineering·Jan HansmannCornelia Kasper
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Enrique Mancha SánchezJ Blas Pagador
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Oct 17, 2019·International Journal of Molecular Sciences·Srikumar KrishnamoorthyChangxue Xu
Jun 12, 2020·Frontiers in Bioengineering and Biotechnology·Lise De MoorHeidi Declercq
Oct 2, 2020·Micromachines·Sean BeverungRobert Steward
Nov 10, 2020·Advanced Healthcare Materials·Greeshma RatheeshYin Xiao
Nov 25, 2020·Tissue Engineering and Regenerative Medicine·Jasmeet Kaur Virdi, Prasad Pethe
Nov 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Taotao LiuHuazhe Yang
Feb 2, 2021·Frontiers in Bioengineering and Biotechnology·Marline KirschAntonina Lavrentieva
Nov 1, 2020·Biomaterials·Wissam FarhatAlexandra Fuchs
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Dec 18, 2020·Biomedical Materials·Candan Yilmaz OzdoganSahin Alagoz
Jul 16, 2020·Biotechnology and Bioengineering·Carola SchmitzAntonina Lavrentieva
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Feb 10, 2021·Tissue Engineering. Part B, Reviews·Marcus Alonso Cee WilliamsDeok-Ho Kim
Apr 3, 2021·Biomaterials·Alessandro PirosaRocky S Tuan

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Methods Mentioned

BETA
bioprinters
rheology
bioprinting
flow cytometry
bioprinter

Software Mentioned

CellSens
GelMA

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