Abstract
This characteristics of adipose-derived mesenchymal stem cells (Ad-MSCs) can be selectively enhanced by altering the culture environment. We evaluated the effects of gelatin on Ad-MSCs when used in combination with culture media. Ad-MSCs were initially cultured in 0%, 0.5%, 1%, 2%, and 4% gelatin in Dulbecco's modified Eagle's medium (DMEM) to evaluate cell proliferation. This expression of inflammatory, antiinflammatory, antioxidant, and osteogenic markers was then assessed by rtPCR in Ad-MSCs cultured in 0.5% gelatin in DMEM (GMSCs), and without gelatin (MSCs), for 5 and 10 days. We found that 0.5% gelatin significantly increased the proliferation rate of Ad-MSCs after 24 h of incubation, up until 72 h. GMSCs had upregulated IL-10, VEGF, and HO-1 after 5 and 10 days of incubation, while IL-6 and TNF-α were upregulated after 5 days and then significantly decreased after 10 days of incubation. The osteogenic factors BMP-7, AXIN, and β-catenin were significantly upregulated in GMSCs after 5 and 10 days. Notably, there was 5- to 8-fold higher expression of BMP-7 in GMSCs than in MSCs. We conclude that culture medium containing 0.5% gelatin enhances the proliferation rate, induces immunosuppression, and activates BMP-7 and the wnt...Continue Reading
References
Jan 1, 1983·Immunological Communications·P ChienA D Schreiber
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 19, 2003·Biomaterials·Ulrich HerselHorst Kessler
Dec 29, 2004·Biomaterials·Yoshitake TakahashiYasuhiko Tabata
Aug 20, 2005·Stem Cells·Panagiota A SotiropoulouMichael Papamichail
Aug 27, 2005·Stem Cells·Mauro KramperaFrancesco Annunziato
Oct 18, 2005·Biomaterials·Feng ZhaoWilliam W Lu
Apr 3, 2008·Journal of Neuroscience Research·Ignazio BarbagalloRoberto Motterlini
May 12, 2009·Journal of Thrombosis and Haemostasis : JTH·H-H LinL-Y Chau
Oct 27, 2009·Bone·Luca VanellaNader G Abraham
Nov 28, 2009·Current Stem Cell Research & Therapy·António J Braga Osório Gomes SalgadoJeffrey M Gimble
Dec 10, 2009·Cell Transplantation·N M VieiraM Zatz
Feb 6, 2010·Expert Opinion on Biological Therapy·Heesang SongKi-Chul Hwang
Jun 23, 2010·Biomaterials·Jiashing YuRandall J Lee
Jul 14, 2010·International Immunopharmacology·Martin J HoogduijnMarc-H Dahlke
Sep 29, 2011·American Journal of Reproductive Immunology : AJRI·Ehsan SoleymaninejadianEsmaeil Samadian
Feb 22, 2012·Cytotherapy·Sung-Su ParkHo Jae Han
Apr 27, 2012·Stem Cells and Development·Paul WaszakBernard Thébaud
Aug 2, 2012·American Journal of Veterinary Research·Agatha H KisielRodolfo Nino-Fong
Aug 15, 2012·Journal of Materials Science. Materials in Medicine·Eva HochKirsten Borchers
Mar 19, 2013·Stem Cell Research & Therapy·Luca VanellaNader G Abraham
Oct 1, 2013·Stem Cell Research & Therapy·Fabian Langenbach, Jörg Handschel
Oct 4, 2014·Science·Hans CleversRoel Nusse
Oct 10, 2014·International Journal of Molecular Sciences·Niels A J CremersFrank A D T G Wagener
May 20, 2015·Seminars in Cell & Developmental Biology·Sergei Y Sokol
Jun 3, 2015·Tissue Engineering. Part a·Liliana F MellorElizabeth G Loboa
Aug 22, 2015·Journal of Orthopaedic Trauma·Joel C WilliamsMark A Lee
Oct 13, 2015·The International Journal of Biochemistry & Cell Biology·Nanmei LiuJinyuan Zhang
Feb 11, 2016·Tissue Engineering. Part B, Reviews·Bjarke FollinAnnette Ekblond
Sep 13, 2016·Oncogene·T ZhanM Boutros
Oct 12, 2016·Cell Transplantation·Ah Young KimOh-Kyeong Kweon
Apr 19, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Saeid KargozarFrancesco Baino