Gelatin sponge-supported histoculture of human nasal mucosa

In Vitro Cellular & Developmental Biology. Animal
K SchierhornG Kunkel

Abstract

Considerable progress has recently been made in the understanding of airway inflammation by cell culture assays and in vivo provocation studies. Inasmuch as ethical considerations limit experimental work in humans, physiologically relevant in vitro models are required to better understand cellular and molecular tissue interactions in human nasal mucosa. Here we describe a human nasal mucosa culture model utilizing a simple gelatin sponge-supported histoculture system at the air-liquid interface. Viable mucosa was preserved for at least 48 h, as shown by morphology and immunohistochemical staining with Ki-67 as marker for proliferation. Pro-inflammatory mediators (kinins, histamine, thromboxane B2, prostaglandin F2 alpha, and substance P) are detectable in serum-containing as well as serum-free culture medium. Incubation with 10(-8) M substance P increases the number of degranulated mast cells after 48 h by 26% (P < 0.01). In this model, biochemical responses can be correlated with histologic alterations of the target tissue. Inflammatory parameters can be examined and compared in various patient groups and different stimulators/inhibitors. This culture method provides a valuable research tool for analyzing all compartments pres...Continue Reading

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Citations

May 5, 1999·American Journal of Respiratory Cell and Molecular Biology·K SchierhornG Kunkel
May 26, 1999·American Journal of Respiratory Cell and Molecular Biology·M V FanucchiJ A Hotchkiss
Jan 10, 2012·The Journal of Allergy and Clinical Immunology·Koji SugawaraRalf Paus
Jun 10, 2008·The Veterinary Journal·Annelies VandekerckhoveHans J Nauwynck
Feb 14, 2013·International Forum of Allergy & Rhinology·Daniel CanteroSarah Vreugde
Mar 5, 2013·The Journal of Allergy and Clinical Immunology·Koji SugawaraRalf Paus
Apr 4, 2021·International Journal of Molecular Sciences·Mika Yamanaka-TakaichiDaisuke Tsuruta

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