Gelatinase B [matrix metalloproteinase (MMP)-9] and collagenases (MMP-8/-13) are upregulated in cerebrospinal fluid during aseptic and bacterial meningitis in children

Neuropathology and Applied Neurobiology
R L LindbergD Leppert

Abstract

We investigated the protein expression of gelatinases [matrix metalloproteinase (MMP)-2 and -9] and collagenases (MMP-8 and -13) in cerebrospinal fluid (CSF) from patients with bacterial (BM, n = 17) and aseptic (AM, n = 14) meningitis. In both, MMP-8 and -9 were increased in 100% of patients, whereas MMP-13 was detectable in 53% and 82% respectively. Three patients with clinical signs of meningitis, without CSF pleocytosis, scored positive for all three MMPs. MMP-8 appeared in two isoforms, granulocyte-type [polymorphonuclear cell (PMN)] and fibroblast/macrophage (F/M) MMP-8. Analysis of kinetic changes from serial lumbar punctures showed that these MMPs are independently regulated, and correlate only partly with CSF cytosis or levels of the endogenous inhibitor, tissue inhibitor of matrix metalloproteinase-1. In vitro, T cells, peripheral blood mononuclear cells (PBMCs) and granulocytes (PMN) release MMP-8 and -9, whereas MMP-13 could be found only in the former two cell types. Using models of exogenous (n-formyl-Met-Leu-Phe, T cell receptor cross-linking) and host-derived stimuli (interleukin-2), the kinetics and the release of the MMP-8, -9 and -13 showed strong variation between these immune cells and suggest release from ...Continue Reading

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Citations

Jun 6, 2006·BMC Biology·Roney S CoimbraStephen L Leib
Jun 19, 2009·The Journal of Infection·Ali A El-SolhJeanine P Wiener-Kronish
Apr 12, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ting-Yen ChiangShun-Fa Yang
Apr 6, 2007·Muscle & Nerve·Susanne Renaud, David Leppert
Jun 7, 2019·Cellular and Molecular Life Sciences : CMLS·Lukas MuriStephen L Leib
Jun 24, 2010·Scandinavian Journal of Gastroenterology·Timo RathJürgen Graf

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