Gelatinases promote calcification of vascular smooth muscle cells by up-regulating bone morphogenetic protein-2

Biochemical and Biophysical Research Communications
Yong-Gang ZhaoRui-Juan Xiu

Abstract

Matrix metalloproteinase-2 (MMP-2), also known as gelatinase A, is involved in vascular calcification. Another member of gelatinases is MMP-9 (gelatinase B). However, the role of gelatinases in the pathogenesis of vascular calcification is not well understood. The current study aims to clarify the relationship between gelatinases and vascular calcification and to elucidate the underlying mechanism. Beta-glycerophosphate (β-GP) was used to induce calcification of vascular smooth muscle cells (VSMCs) with or without 2-[[(4-Phenoxyphenyl)sulfonyl]methyl]-thiirane (SB-3CT), a specific gelatinases inhibitor. Levels of calcification were determined by assessing calcium content and calcification area of VSMCs. Phenotype transition of VSMCs was observed by assessing expressions of alkaline phosphatase (ALP), smooth muscle α-actin (SM-α-actin) and desmin. Gelatin zymography was applied to determine the activities of gelatinases, and western blot was applied to determine expressions of gelatinases, bone morphogenetic protein-2 (BMP-2), Runt-related transcription factor 2 (RUNX2) and msh homeobox homolog 2 (Msx-2). Gelatinases inhibition by SB-3CT alleviated calcification and phenotype transition of VSMCs induced by β-GP. Increased gelati...Continue Reading

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Citations

Oct 30, 2016·Biochemical and Biophysical Research Communications·Tianjia LiChangwei Liu
Jun 22, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ralf G RempeBjörn Bauer
Jan 3, 2019·Interactive Cardiovascular and Thoracic Surgery·Arkady RutkovskiyAnna Malashicheva
Feb 7, 2017·BioMed Research International·Wenhong JiangXiao Qin
Oct 23, 2020·Journal of Cellular and Molecular Medicine·Shan-Shan WangHan Chen
May 11, 2021·Frontiers in Cardiovascular Medicine·Wenhong JiangXiao Qin
Jul 3, 2021·Journal of Personalized Medicine·Yana V PolonskayaYulia I Ragino

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