Gelatin/Hyaluronic Acid Content in Hydrogels Obtained through Blue Light-Induced Gelation Affects Hydrogel Properties and Adipose Stem Cell Behaviors

Biomolecules
Shinji SakaiMasahito Taya

Abstract

Composite hydrogels of hyaluronic acid and gelatin attract great attention in biomedical fields. In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering. In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels. Control of the content enabled gelation in less than 20 s, and also enabled hydrogels to be obtained with 0.5-1.2 kPa Young's modulus. Human adipose stem cells were more elongated in hydrogels with a higher rather than lower content of hyaluronic acid. Stem cell marker genes, Nanog, Oct4, and Sox2, were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes. These results are useful for designing conditions for using gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications.

References

Jan 1, 1993·Critical Reviews in Biotechnology·J HuaL A Glasgow
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·D A Fancy, T Kodadek
Mar 17, 2005·International Journal of Biological Macromolecules·J G PrietoA I Alvarez
Mar 3, 2007·Biotechnology Letters·Shinji SakaiKoei Kawakami
Jul 3, 2008·Methods : a Companion to Methods in Enzymology·Bruce A BunnellCynthia Ripoll
May 20, 1996·Biotechnology and Bioengineering·R H LiF T Gentile
Jun 29, 2010·Acta Biomaterialia·Tae Il SonYoshihiro Ito
Apr 11, 2012·Journal of Tissue Engineering and Regenerative Medicine·Yuko OgushiKoei Kawakami
Feb 20, 2013·Biomacromolecules·Gulden Camci-UnalAli Khademhosseini
Mar 19, 2014·Nature Materials·Chun YangKristi S Anseth
Jun 16, 2015·Frontiers in Immunology·Margot Zöller
Jun 23, 2015·Macromolecular Bioscience·Khoon S LimPenny J Martens
Jun 23, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Spencer L Fenn, Rachael A Oldinski
Jul 15, 2015·Biotechnology Letters·Kai Su, Chunming Wang
Feb 20, 2016·International Journal of Biological Macromolecules·Mahadevappa HemshekharKesturu S Girish
Jun 10, 2016·International Journal of Immunopathology and Pharmacology·Manuele CasaleFabrizio Salvinelli
Nov 7, 2017·Carbohydrate Polymers·Anuj KumarSung Soo Han
Nov 25, 2017·Nature Materials·Linqing LiChristopher S Chen
Dec 8, 2017·ACS Omega·Vladimíra MoulisováGloria Gallego Ferrer
Dec 23, 2017·BMC Musculoskeletal Disorders·Andrew ConcoffJeffrey Rosen
May 12, 2016·Bioactive Materials·Yung-Hao TsouXiaoyang Xu
Aug 9, 2018·Materials·Mahboobeh RezaeeyazdiSidi A Bencherif
Oct 10, 2016·ACS Biomaterials Science & Engineering·Liliang OuyangJason A Burdick

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