Geldanamycin-induced phosphatidylserine translocation in the erythrocyte membrane

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Kashif JilaniFlorian Lang

Abstract

Geldanamycin, a benzoquinone ansamycin antibiotic, and its analogues induce apoptosis of tumor cells and are thus considered for the treatment of cancer. Similar to apoptosis of nucleated cells, erythrocytes may enter eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and by cell membrane scrambling with phosphatidylserine-exposure at the erythrocyte surface. Triggers of eryptosis include increase of cytosolic Ca(2+)-concentration ([Ca(2+)]i) and formation of ceramide. The present study explored, whether geldanamycin modifies [Ca(2+)]i, ceramide formation, cell volume and phosphatidylserine abundance at the erythrocyte surface. Erythrocyte volume was estimated from forward scatter, phosphatidylserine-abundance from annexin V binding, hemolysis from hemoglobin release, ceramide formation from binding of fluorescent antibodies and [Ca(2+)]i from Fluo3-fluorescence. A 48 hours exposure to geldanamycin significantly decreased forward scatter (≥ 5 µM), significantly increased annexin-V-binding (≥ 25 µM), but did not significantly modify Fluo3-fluorescence (up to 50 µM). The annexin-V-binding following geldanamycin treatment was not significantly modified by removal of extracellular Ca(2+) but was paralleled by...Continue Reading

Citations

Mar 22, 2014·Toxins·Leonie FrauenfeldFlorian Lang
May 27, 2015·Toxins·Rosi BissingerFlorian Lang
Apr 29, 2015·Toxins·Abaid MalikFlorian Lang
Dec 24, 2014·Toxins·Kousi AlzoubiFlorian Lang
Apr 20, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Majed AbedFlorian Lang
Feb 1, 2015·Apoptosis : an International Journal on Programmed Cell Death·Elisabeth LangFlorian Lang
Jun 14, 2014·International Journal of Hematology·Rosi BissingerFlorian Lang
Jun 5, 2014·Basic & Clinical Pharmacology & Toxicology·Abaid MalikFlorian Lang
Aug 20, 2014·Basic & Clinical Pharmacology & Toxicology·Kousi AlzoubiFlorian Lang
Nov 15, 2014·Basic & Clinical Pharmacology & Toxicology·Salvatrice CalabròFlorian Lang
Oct 29, 2014·Basic & Clinical Pharmacology & Toxicology·Salvatrice CalabròFlorian Lang
Mar 31, 2015·BioMed Research International·Elisabeth Lang, Florian Lang
May 28, 2015·Expert Opinion on Therapeutic Targets·Florian LangElisabeth Lang
Jan 28, 2020·Dose-response : a Publication of International Hormesis Society·Sajida IlyasAsma Asghar
Aug 19, 2015·Basic & Clinical Pharmacology & Toxicology·Thomas PeterFlorian Lang

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